Draft genome sequence of Clostridium jeddahense EE-R19 isolated from an anaerobic digester
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(2020) 13:413 Ziganshina et al. BMC Res Notes https://doi.org/10.1186/s13104-020-05247-3
Open Access
DATA NOTE
Draft genome sequence of Clostridium jeddahense EE‑R19 isolated from an anaerobic digester Elvira E. Ziganshina1, Waleed S. Mohammed2 and Ayrat M. Ziganshin1*
Abstract Objectives: Clostridium species of the order Clostridiales are mostly strictly anaerobic rod-shaped bacteria. They can be detected in a variety of environments, including the intestines of humans and animals, soil, water, and biogas reactors. Species of the genus Clostridium are widely used in various biotechnological processes, but several of them have been identified as significant human pathogens. Therefore, investigation at the genome level is necessary to provide valuable information about the ecology, genetics, and phylogenetic diversity of various Clostridium species. Data description: In the present study, we report the whole genome sequence of Clostridium jeddahense strain EE-R19, which was isolated from a mesophilic anaerobic digester. The draft genome of C. jeddahense EE-R19 consisted of 59 contigs (> 500 bp), which amounted to 3,562,974 with an overall G + C content of 51.79%. The whole genome shotgun project of C. jeddahense EE-R19 has been deposited at DDBJ/ENA/GenBank under the accession number JAAVNF000000000. Keywords: Draft genome, Firmicutes, Clostridium jeddahense, Anaerobic digester Objective Members of the order Clostridiales can be found in a variety of environments, including the intestines of humans and animals, soil, water, marine environments, and biogas reactors [1–4]. The clostridia are basically strict anaerobes with gram-positive staining and the ability to sporulate. Their metabolism varies greatly because they can metabolize various compounds, including carbohydrates, proteins, alcohols, amino acids, and purines. Organic acids and alcohols can be obtained during the metabolism of carbohydrates or proteins. However, some of them have been identified as human pathogens [1]. The genome data will expand understanding of the *Correspondence: [email protected] 1 Department of Microbiology, Institute of Fundamental Medicine and Biology, Kazan (Volga Region) Federal University, Kremlyovskaya Str. 18, Kazan 420008, Russia Full list of author information is available at the end of the article
genus Clostridium and provide valuable information about ecology and genetics of the species Clostridium jeddahense. Data description
Clostridium jeddahense strain EE-R19 was originally isolated from anaerobically digested distillers grains and cow manure at mesophilic temperatures on nutrient agar plates in a BACTRON anaerobic chamber (Shel Lab) in Kazan, Republic of Tatarstan, Russia. The strain C. jeddahense EE-R19 was then cultivated on nutrient agar plates at + 38 °C for 72 h in a BACTRON anaerobic chamber. Biomass from a culture of C. jeddahense EE-R19 was harvested, washed twice with sterile K-Na-phosphate buffer (pH 7.0), and genomic DNA was extracted by using FastDNA spin kit (MP Biomedicals) according
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