Genome-wide analysis of laccase genes in moso bamboo highlights PeLAC10 involved in lignin biosynthesis and in response
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ORIGINAL ARTICLE
Genome‑wide analysis of laccase genes in moso bamboo highlights PeLAC10 involved in lignin biosynthesis and in response to abiotic stresses Lichao Li1 · Kebin Yang1 · Sining Wang1 · Yongfeng Lou2 · Chenglei Zhu1 · Zhimin Gao1 Received: 17 October 2019 / Accepted: 26 February 2020 © Springer-Verlag GmbH Germany, part of Springer Nature 2020
Abstract Key message Twenty-three PeLACs have been identified in moso bamboo, overexpression of PeLAC10 increases the lignin content and confers drought and phenolic acid tolerance in transgenic Arabidopsis. Abstract Laccases (LACs) have multifunction involved in the processes of cell elongation, lignification and stress response in plants. However, the function of laccases in bamboo remain unclear. Here, a total of 23 laccase genes (PeLAC1–PeLAC23) were identified in moso bamboo (Phyllostachys edulis). The diverse gene structure and expression pattern of PeLACs suggested that their function should be spatiotemporal and complicated, which was supported by the expression profiles in different tissues of moso bamboo. Eighteen PeLACs were identified as the targets of ped-miR397. The putative pedmiR397-binding site in the coding region of PeLAC10 was further confirmed by RLM-5′ RACE, indicating that PeLAC10 was regulated by ped-miR397 after transcription. With the increasing shoot height, the expression abundance of PeLAC10 was up-regulated and reached the maximum in 15 cm shoots, while that of ped-miR397 was relative lower and showed the minimum in 15 cm shoots. PeLAC10 was up-regulated obviously under both ABA (100 μmol L–1) and NaCl (400 mmol L–1) treatments, and it was down-regulated under the GA3 (100 μmol L–1) treatment. The transgenic Arabidopsis plants overexpressing PeLAC10 became slightly smaller and their petioles were shorter than those of Col-0. However, they had a stronger capacity in resistance to phenolic acids and drought besides higher lignin content in stems. These results indicated that overexpression of PeLAC10 was helpful to increase the content of lignin in transgenic Arabidopsis and improve the adaptability to phenolic acid and drought stresses. Keywords Phyllostachys edulis · Laccase gene · Lignin biosynthesis · Abiotic stresses
Communicated by Hiroyasu Ebinuma. Lichao Li and Kebin Yang these authors contributed equally to this work. Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00299-020-02528-w) contains supplementary material, which is available to authorized users. * Zhimin Gao [email protected] 1
National Forestry and Grassland Administration, Beijing Key Open Laboratory On the Science and Technology of Bamboo and Rattan, Institute of Gene Science and Industrialization for Bamboo and Rattan Resources, International Center for Bamboo and Rattan, Beijing 100102, China
Jiangxi Academy of Forestry, Nanchang 330013, China
2
Abbreviations 4CL 4-(Hydroxy) cinnamoyl CoA ligase C3H p-coumarate 3-hydroxylase C4H Cinnamate 4-hydroxylase CAD Cinnamyl alcohol dehydrogenase C
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