Genomic and phenotypic analysis of siderophore-producing Rhodococcus qingshengii strain S10 isolated from an arid weathe
- PDF / 840,714 Bytes
- 6 Pages / 595.276 x 790.866 pts Page_size
- 68 Downloads / 174 Views
SHORT COMMUNICATION
Genomic and phenotypic analysis of siderophore‑producing Rhodococcus qingshengii strain S10 isolated from an arid weathered serpentine rock environment Irina V. Khilyas1 · Alyona V. Sorokina1 · Maria I. Markelova1 · Maksim Belenikin2 · Lilia Shafigullina1 · Rezeda I. Tukhbatova3 · Elena I. Shagimardanova4 · Jochen Blom5 · Margarita R. Sharipova1 · Michael F. Cohen6 Received: 13 April 2020 / Revised: 8 July 2020 / Accepted: 17 September 2020 © Springer-Verlag GmbH Germany, part of Springer Nature 2020
Abstract The success of members of the genus Rhodococcus in colonizing arid rocky environments is owed in part to desiccation tolerance and an ability to extract iron through the secretion and uptake of siderophores. Here, we report a comprehensive genomic and taxonomic analysis of Rhodococcus qingshengii strain S10 isolated from eathered serpentine rock at the arid Khalilovsky massif, Russia. Sequence comparisons of whole genomes and of selected marker genes clearly showed strain S10 to belong to the R. qingshengii species. Four prophage sequences within the R. qingshengii S10 genome were identified, one of which encodes for a putative siderophore-interacting protein. Among the ten non-ribosomal peptides synthase (NRPS) clusters identified in the strain S10 genome, two show high homology to those responsible for siderophore synthesis. Phenotypic analyses demonstrated that R. qingshengii S10 secretes siderophores and possesses adaptive features (tolerance of up to 8% NaCl and pH 9) that should enable survival in its native habitat within dry serpentine rock. Keywords Rhodococcus qingshengii · Desiccation · Siderophores · Serpentinite · Phenotype · Pan-genome Members of the genus Rhodococcus (phylum Actinobacteria, family Nocardiaceae) can degrade and transform a Communicated by Erko Stackebrandt. * Irina V. Khilyas [email protected] 1
Department of Microbiology, Institute of Fundamental Medicine and Biology, Kazan (Volga Region), Federal University, Kazan, Russian Federation
2
Department of Molecular and Biological Physics, Moscow Institute of Physics and Technology (State University), Dolgoprudny, Russia
3
Laboratory of Structural Biology, Institute of Fundamental Medicine and Biology, Kazan (Volga Region), Federal University, Kazan, Russian Federation
4
Laboratory of Extreme Biology, Institute of Fundamental Medicine and Biology, Kazan (Volga Region), Federal University, Kazan, Russian Federation
5
Bioinformatics and Systems Biology, Justus-Liebig-University Giessen, Giessen, Germany
6
Department of Biology, Sonoma State University, Rohnert Park, CA, USA
range of environmental pollutants or synthesize compounds with commercially useful applications (Christofi and Ivshina 2002). Over the past 20 years, rhodococci have found predominant use as a bioremediation and bioconversion tool with relatively little attention given to their potential for biosynthesis of natural products for use in the pharmaceutical and agricultural sectors (Ceniceros et al. 2017). Along t
Data Loading...
