Genomic analysis of Helicobacter himalayensis sp. nov. isolated from Marmota himalayana
- PDF / 3,145,154 Bytes
- 10 Pages / 595.276 x 790.866 pts Page_size
- 21 Downloads / 173 Views
RESEARCH ARTICLE
Open Access
Genomic analysis of Helicobacter himalayensis sp. nov. isolated from Marmota himalayana Shoukui Hu1†, Lina Niu2†, Lei Wu1†, Xiaoxue Zhu1†, Yu Cai1, Dong Jin3, Linlin Yan1* and Fan Zhao1*
Abstract Background: Helicobacter himalayensis was isolated from Marmota himalayana in the Qinghai-Tibet Plateau, China, and is a new non-H. pylori species, with unclear taxonomy, phylogeny, and pathogenicity. Results: A comparative genomic analysis was performed between the H. himalayensis type strain 80(YS1)T and other the genomes of Helicobacter species present in the National Center for Biotechnology Information (NCBI) database to explore the molecular evolution and potential pathogenicity of H. himalayensis. H. himalayensis 80(YS1)T formed a clade with H. cinaedi and H. hepaticus that was phylogenetically distant from H. pylori. The H. himalayensis genome showed extensive collinearity with H. hepaticus and H. cinaedi. However, it also revealed a low degree of genome collinearity with H. pylori. The genome of 80(YS1)T comprised 1,829,936 bp, with a 39.89% GC content, a predicted genomic island, and 1769 genes. Comparatively, H. himalayensis has more genes for functions in “cell wall/membrane/envelope biogenesis” and “coenzyme transport and metabolism” sub-branches than the other compared helicobacters, and its genome contained 42 virulence factors genes, including that encoding cytolethal distending toxin (CDT). Conclusions: We characterized the H. himalayensis 80(YS1)T genome, its phylogenetic position, and its potential pathogenicity. However, further understanding of the pathogenesis of this potentially pathogenic bacterium is required, which might help to manage H. himalayensis-induced diseases. Keywords: Helicobacter, Comparative genomics, Helicobacter himalayensis, Virulence factor
Background The Helicobacter genus comprises gram-negative bacteria with characteristic spiral shapes [1]. The type species, Helicobacter pylori, was the first cultivated species of the genus. H. pylori was first isolated from in the stomachs of patients with gastritis and peptic ulceration in 1984 [2]. Currently, over 30 species have been isolated, including many candidate or unclassified species [3]. * Correspondence: [email protected]; [email protected] † Shoukui Hu, Lina Niu, Lei Wu and Xiaoxue Zhu contributed equally to this work. 1 Department of Clinical Laboratory, Peking University Shougang Hospital, Beijing 100144, China Full list of author information is available at the end of the article
Except for H. pylori, most of the other Helicobacter have been isolated and identified from wild animals, including rodents, cats, dogs, rabbits, chickens, sheep, cattle, swine, cheetahs, ferrets, dolphins, whales, and non-human primates [4–8]. In addition, certain Helicobacters were isolated from laboratory animals [7, 9–11]. Convincing evidence has identified H. pylori as a causative bacterium of gastric adenocarcinoma, mucosa-associated lymphoid tissue lymphoma, and peptic ulcer in humans [12]. However, studies of
Data Loading...
