Characterization and genomic analysis of a diesel-degrading bacterium, Acinetobacter calcoaceticus CA16, isolated from C
- PDF / 1,819,972 Bytes
- 15 Pages / 595.276 x 790.866 pts Page_size
- 4 Downloads / 211 Views
RESEARCH ARTICLE
Open Access
Characterization and genomic analysis of a diesel-degrading bacterium, Acinetobacter calcoaceticus CA16, isolated from Canadian soil Margaret T. Ho1,2, Michelle S. M. Li1, Tim McDowell3, Jacqueline MacDonald1 and Ze-Chun Yuan1,3*
Abstract Background: With the high demand for diesel across the world, environmental decontamination from its improper usage, storage and accidental spills becomes necessary. One highly environmentally friendly and cost-effective decontamination method is to utilize diesel-degrading microbes as a means for bioremediation. Here, we present a newly isolated and identified strain of Acinetobacter calcoaceticus (‘CA16’) as a candidate for the bioremediation of diesel-contaminated areas. Results: Acinetobacter calcoaceticus CA16 was able to survive and grow in minimal medium with diesel as the only source of carbon. We determined through metabolomics that A. calcoaceticus CA16 appears to be efficient at diesel degradation. Specifically, CA16 is able to degrade 82 to 92% of aliphatic alkane hydrocarbons (CnHn + 2; where n = 12–18) in 28 days. Several diesel-degrading genes (such as alkM and xcpR) that are present in other microbes were also found to be activated in CA16. Conclusions: The results presented here suggest that Acinetobacter strain CA16 has good potential in the bioremediation of diesel-polluted environments. Keywords: Microbial bioremediation, Acinetobacter calcoaceticus CA16, Diesel-degrading bacteria, Diesel bioremediation, Aliphatic hydrocarbons, n-alkanes
Background With the high demand for diesel around the world, severe environmental and ecological problems have arisen from its improper usage, storage and disposal, as well as accidental leakage. Diesel oil, a very complex mixture of hydrocarbons (e.g. aliphatics, aromatics, alcohols), and its byproducts, are known soil contaminants and are phytotoxic to a wide variety of plants [1, 2]. Such effects * Correspondence: [email protected]; [email protected] 1 Department of Microbiology and Immunology, The University of Western Ontario, 1151 Richmond Street, London, Ontario N6A 5B7, Canada 3 London Research and Development Centre, Agriculture and Agri-Food Canada, 1391 Sandford Street, London, Ontario N5V 4T3, Canada Full list of author information is available at the end of the article
can be mitigated by microbial bioremediation, which uses microbes to remove pollutants from the environment [3, 4]. In particular, bioaugmentation involves the addition of living bacteria to a contaminated site, while biostimulation adds supplemental nutrients to existing on-site bacteria with the goal of optimizing their metabolism [5]. On-site bioremediation is considered one of the cheapest and least laborious methods to remove unwanted hydrocarbons from contaminated ecosystems, and it is also effective and environmentally friendly [6]. Sites can be pre-screened for diesel-degrading bacterial candidates that can promote plant growth [7] and
© The Author(s). 2020 Open Access This article is licensed under a Creative Co
Data Loading...
