Genotyping and phylogenetic analysis of hydatid cysts isolated from livestock in Bushehr province, Iran
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ORIGINAL ARTICLE
Genotyping and phylogenetic analysis of hydatid cysts isolated from livestock in Bushehr province, Iran Zahra Babaei1 • Reza Taherkhani2 • Afshin Barazesh3 • Marzieh Taherzadeh4 Soleiman Khorami4 • Moradali Fouladvand5
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Received: 23 July 2020 / Accepted: 13 October 2020 Ó Indian Society for Parasitology 2020
Abstract Hydatid cyst is one of the parasitic zoonoses caused by infection with the larval stage of Echinococcus granulosus tapeworm. The spread of this parasite is global and is of great importance in terms of public health. To date, ten different species of this parasite have been identified that differ in characteristics such as life cycle, epidemiology and pathogenesis. The purpose of this study was to determine the genotype and phylogenetic relationship of hydatid cysts isolated from livestock of Bushehr province, Iran. About 62 samples of hepatic and pulmonary hydatid cysts were collected from slaughtered animals. DNA extracted by phenol–chloroform method was amplified by PCR using primers specific for the cox1 gene. The PCR products of 50 samples were sequenced and analyzed using BioEdit software and compared with sequences in the GenBank. The phylogenetic tree was drawn using Neighbor Joining tree-NJ method, and its reliability was evaluated. Sequencing results showed that out of 50 sequenced & Moradali Fouladvand [email protected] 1
Persian Gulf Hospital, Faculty of Medicine, Bushehr University of Medical Sciences, Bushehr, Iran
2
Department of Virology, Faculty of Medicine, Bushehr University of Medical Sciences, Bushehr, Iran
3
The Persian Gulf Marine Biotechnology Research Center, The Persian Gulf Biomedical Sciences Research Institute, Bushehr University of Medical Sciences, Bushehr, Iran
4
Department of Laboratory Sciences, Faculty of Paramedical Sciences, Bushehr University of Medical Sciences, Bushehr, Iran
5
Department of Microbiology and Parasitology, The Persian Gulf Tropical and Infectious Diseases Research Center, Bushehr University of Medical Sciences, Salman Farsi St, Bushehr, Iran
samples, 43 samples had the genotype of Echinococcus granulosus and 7 samples had the genotype of Taenia hydatigena. By drawing a phylogenetic tree, all 43 hydatid cyst samples belonged to G1 strain. The predominance of G1 strain of hydatid cyst in livestock of Bushehr province shows the main role of this genotype in establishing the life cycle of parasite in this region and if the genotype of the parasite in dogs and humans is determined, then these findings can be used to disrupt the life cycle of the parasite and reduce the human infections. Keywords Genotype Hydatid cyst Echinococcus granulosus Bushehr province
Introduction Hydatid disease or hydatidosis is one of the most important parasitic zoonoses caused by infection with the larval stage of Echinococcus granulosus tapeworm (Thompson 2017). In the life cycle of this parasite, Canidae, especially herding dogs and street dogs, plays the role of the final hosts and infect the pastures and vegetable fields by rep
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