Gonadotropin-Releasing Hormone Receptor Expression in Human Spinal Cord
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Gonadotropin‑Releasing Hormone Receptor Expression in Human Spinal Cord Carmen Díaz‑Galindo1 · Denisse Calderón‑Vallejo1,2 · Irma Hernández‑Jasso1 · Daniel Cervantes‑García3 · Diego Martínez‑Díaz1 · David Ibarra‑Martínez2 · Martín Muñoz‑Ortega4 · J. Luis Quintanar1 Received: 7 August 2020 / Revised: 3 November 2020 / Accepted: 11 November 2020 © Springer Science+Business Media, LLC, part of Springer Nature 2020
Abstract The expression of the gonadotrophin-releasing hormone receptor expression on pituitary gonadotrophs in humans is well characterized. In nervous system they have also been found in hippocampi and cerebral cortex. However, gonadotrophinreleasing hormone receptor expression in human spinal cord has not been reported. This study was to analyze the gonadotrophin-releasing hormone receptor expression in human spinal cord by immunohistochemistry, mRNAs by reverse transcriptase polymerase chain reaction, cDNA cloning and Western blot. The results show immunoreactive material to gonadotrophin-releasing hormone receptor in motoneurons of the spinal cord. Further, the study revealed that spinal cord expressed the gonadotrophin-releasing hormone receptor mRNA. The amplicon sequence corresponds to 100% of identity to GenBank. In Western blot, a band of 37 kDa were found in extracts of spinal cord and placenta as a control. In conclusion, human spinal cord expresses gonadotrophin-releasing hormone receptor analyzed through immunohistochemistry, the expression of its mRNA, cloning its cDNA and Western blot analysis. The presence of gonadotrophin-releasing hormone receptor in the spinal cord suggests the possibility of an extrapituitary functional role independent of reproductive system. Keywords GnRH · Human GnRH-R · Human spinal cord · Immunohistochemistry · RT-PCR
Introduction It has been well established that in mammals, the hypothalamic decapeptide gonadotrophin-releasing hormone (GnRH) binds to high affinity receptors on pituitary gonadotrophs [1, 2]. These receptors (termed type I) mediate the effects of GnRH on synthesis and secretion of * J. Luis Quintanar [email protected] 1
Department of Physiology and Pharmacology, Center of Basic Sciences, Universidad Autónoma de Aguascalientes, Av. Universidad 940, Col. Ciudad Universitaria, CP 20131 Aguascalientes, AGS, Mexico
2
Department of Morphology, Center of Basic Sciences, Universidad Autónoma de Aguascalientes, Aguascalientes, Mexico
3
Department of Microbiology, Center of Basic Sciences, Universidad Autónoma de Aguascalientes, Aguascalientes, Mexico
4
Department of Chemistry, Center of Basic Sciences, Universidad Autónoma de Aguascalientes, Aguascalientes, Mexico
gonadotropins, luteinizing hormone (LH) and follicle stimulating hormone (FSH) [3, 4]. However, GnRH may have other extra-pituitary roles in the central nervous system. This fact is strongly suggested by studies showing the presence of GnRH receptor (GnRH-R) in different areas of the nervous system, such as cerebral cortical neurons in embryos and adu
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