Growth hormone alleviates oxidative stress and improves the IVF outcomes of poor ovarian responders: a randomized contro
- PDF / 1,401,281 Bytes
- 10 Pages / 595.276 x 790.866 pts Page_size
- 81 Downloads / 160 Views
(2020) 18:91
RESEARCH
Open Access
Growth hormone alleviates oxidative stress and improves the IVF outcomes of poor ovarian responders: a randomized controlled trial Yan Gong1* , Kun Zhang2*, Dongsheng Xiong1, Jiajing Wei1, Hao Tan2 and Shengfang Qin3
Abstract Background: Oxidative stress (OS), defined as an imbalance between excessive reactive oxygen species (ROS) and/ or reactive nitrogen species (RNS) production and antioxidant insufficiency, has been suggested to be involved in the pathogenesis of poor ovarian response (POR). Growth hormone (GH) can reduce OS in some cell types. This study investigated whether GH can improve OS and the in vitro fertilization and embryo transfer (IVF-ET) outcomes of poor ovarian responders. Methods: This study enrolled 105 patients with POR and 58 patients without POR (controls) who were diagnosed according to the Bologna criteria and underwent conventional IVF-ET. Poor ovarian responders were randomly assigned to two groups: the POR-GH group, which received pretreatment with GH 4 IU/d on day 2 of the previous menstrual cycle before IVF until the trigger day, and the POR-C group, which received no pretreatment. OS markers in follicular fluid (FF), ROS levels in granulosa cells (GCs), and the IVF outcomes of the groups were compared. Results: Endometrial thickness on trigger day, the number of cleaved embryos, the number of higher-quality embryos, and the rates of embryo formation, higher-quality embryo formation, implantation and clinical pregnancy were significantly increased in the POR-GH group compared with the POR-C group (P < 0.05). Moreover, compared to those in the non-POR group, FF malondialdehyde (MDA), total oxidant status (TOS), oxidative stress index (OSI) and ROS levels in GCs were significantly higher, whereas superoxide dismutase (SOD) and the total antioxidant capacity (TAC) were significantly lower in the POR-C group (P < 0.05). Furthermore, compared with those in the POR-C group, the FF TAC was significantly increased in the POR-GH group, and TOS, OSI and intracellular ROS levels were significantly reduced (P < 0.05). (Continued on next page)
* Correspondence: [email protected]; [email protected] 1 Reproductive Medicine Centre, Sichuan Provincial Women’s and Children’s Hospital, The Affiliated Women’s and children’s Hospital of Chengdu Medical College, #290 Shayan West Second Street, Wuhou District, Chengdu, Sichuan, People’s Republic of China 2 Department of Genetics, School of Bioscience and Technology, Chengdu Medical College, #783 Xindu Avenue, Xindu District, Chengdu, Sichuan 610500, People’s Republic of China Full list of author information is available at the end of the article © The Author(s). 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were
Data Loading...
