Hemodialysis Decreases Serum Brain-Derived Neurotrophic Factor Concentration in Humans
- PDF / 311,174 Bytes
- 10 Pages / 595.276 x 790.866 pts Page_size
- 16 Downloads / 188 Views
ORIGINAL PAPER
Hemodialysis Decreases Serum Brain-Derived Neurotrophic Factor Concentration in Humans Jerzy A. Zoladz • Michał S´migielski • Joanna Majerczak • Łukasz R. Nowak • Justyna Zapart-Bukowska • Olgierd Smolen´ski • Jan Kulpa • Krzysztof Duda • Joanna Drzewin´ska • Grzegorz Bartosz
Received: 27 March 2012 / Revised: 27 July 2012 / Accepted: 30 July 2012 / Published online: 19 August 2012 Ó Springer Science+Business Media, LLC 2012
Abstract In the present study we have evaluated the effect of a single hemodialysis session on the brain-derived neurotrophic factor levels in plasma [BDNF]pl and in serum [BDNF]s as well as on the plasma isoprostanes concentration [F2 isoprostanes]pl, plasma total antioxidant capacity (TAC) and plasma cortisol levels in chronic kidney disease patients. Twenty male patients (age 69.8 ± 2.9 years (mean ± SE)) with end-stage renal disease undergoing maintenance hemodialysis on regular dialysis treatment for 15–71 months participated in this study. A single hemodialysis session, lasting 4.2 ± 0.1 h, resulted in a decrease (P = 0.014) in [BDNF]s by *42 % (2,574 ± 322 vs. 1,492 ± 327 pg ml-1). This was accompanied by an increase (P \ 10-4) of [F2-Isoprostanes]pl (38 ± 3 vs. 116 ± 16 pg ml-1), decrease (P \ 10-4) in TAC (1,483 ± 41
vs. 983 ± 35 trolox equivalents, lmol l-1) and a decrease (P = 0.004) in plasma cortisol level (449.5 ± 101.2 vs. 315.3 ± 196.3 nmol l-1). No changes (P [ 0.05) in [BDNF]pl and the platelets count were observed after a single dialysis session. Furthermore, basal [BDNF]s in the chronic kidney disease patients was significantly lower (P = 0.03) when compared to the age-matched control group (n = 23). We have concluded that the observed decrease in serum BDNF level after hemodialysis accompanied by elevated [F2-Isoprostanes]pl and decreased plasma TAC might be caused by enhanced oxidative stress induced by hemodialysis.
J. A. Zoladz (&) J. Majerczak J. Zapart-Bukowska Department of Physiology and Biochemistry, Faculty of Rehabilitation, University School of Physical Education Krako´w, al. Jana Pawla II 78, 31-571 Krako´w, Poland e-mail: [email protected]
Introduction
M. S´migielski O. Smolen´ski Dialysis Center Fresenius Nephrocare II, Rydygier Hospital, os. Złotej Jesieni 1, 31-826 Krako´w, Poland Ł. R. Nowak J. Kulpa K. Duda Center of Oncology, M. Sklodowska-Curie Memorial Institute, Krako´w Division, ul. Garncarska 11, 31-115 Krako´w, Poland O. Smolen´ski Department of Clinical Rehabilitation, Faculty of Rehabilitation, University School of Physical Education Krako´w, al. Jana Pawla II 78, 31-571 Krako´w, Poland J. Drzewin´ska G. Bartosz Department of Molecular Biophysics, University of Ło´dz, ul. Banacha 12/16, 90-237 Ło´dz, Poland
Keywords Ageing BDNF Kidney disease Isoprostanes Oxidative stress Cortisol
Brain derived neurotrophic factor (BDNF) is the most abundant in the neurotrophin growth factor family [1–3]. It was demonstrated that BDNF expression is present in various parts of the human brain, including the hippo
Data Loading...
