Hepatitis B Virus Methods and Protocols

This volume serves as a reference for the dissemination of advances made in the study of Hepatitis B Virus (HBV). Hepatitis B Virus: Methods and Protocols details protocols and techniques ranging from cell culture studies to in vivo and clinical immunolog

  • PDF / 9,336,990 Bytes
  • 301 Pages / 504.63 x 737.01 pts Page_size
  • 102 Downloads / 235 Views

DOWNLOAD

REPORT


Haitao Guo Andrea Cuconati Editor

Hepatitis B Virus Methods and Protocols

Methods

in

Molecular Biology

Series Editor John M. Walker School of Life and Medical Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK

For further volumes: http://www.springer.com/series/7651

Hepatitis B Virus Methods and Protocols

Edited by

Haitao Guo Department of Microbiology and Immunology, Indiana University School of Medicine, Indianapolis, IN, USA

Andrea Cuconati Arbutus Biopharma, Inc., Doylestown, PA, USA

Editors Haitao Guo Department of Microbiology and Immunology Indiana University School of Medicine Indianapolis, IN, USA

Andrea Cuconati Arbutus Biopharma, Inc. Doylestown, PA, USA

ISSN 1064-3745     ISSN 1940-6029 (electronic) Methods in Molecular Biology ISBN 978-1-4939-6698-1    ISBN 978-1-4939-6700-1 (eBook) DOI 10.1007/978-1-4939-6700-1 Library of Congress Control Number: 2016961703 © Springer Science+Business Media LLC 2017 This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed. The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. The publisher, the authors and the editors are safe to assume that the advice and information in this book are believed to be true and accurate at the date of publication. Neither the publisher nor the authors or the editors give a warranty, express or implied, with respect to the material contained herein or for any errors or omissions that may have been made. Cover Image: HBV infection in HepG2 cells reconstituted with the viral receptor NTCP (HepG2-NTCP). HepG2NTCP cells were infected with HBV, on day 7 post infection, the core antigen of HBV (HBc) was stained with an antiHBc monoclonal antibody (1C10) in green, HBc antigen is distributed both in cell nuclei and cytoplasm. Cell nuclei were stained with DAPI in blue. (modified from Figure 1 in Chapter 1) Printed on acid-free paper This Humana Press imprint is published by Springer Nature The registered company is Springer Science+Business Media LLC The registered company address is: 233 Spring Street, New York, NY 10013, U.S.A.

Preface By any measure, hepatitis B is one of the world’s most important infectious diseases, by which up to one third of the world’s population may have been initially infected, with up to 400 million still suffering a chronic infection. The causative agent is hepatitis B virus (HBV), a virus that straddles the line between DNA and RNA viruses, with a DNA ge