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High-performance multiplex microvalves fabrication and using for tumor cells staining on a microfluidic chip Shao-Li Hong 1,2,3 & Man Tang 1,2,3 & Zhengqi Chen 1,2,3 & Zhao Ai 1,2,3 & Feng Liu 1,2,3,4 & Shuibing Wang 1,2,3 & Nangang Zhang 1,2,3 & Kan Liu 1,2,3,5

# Springer Science+Business Media, LLC, part of Springer Nature 2019

Abstract As we all know, microvalve holds great importance for microfluidic manipulation in chip. Herein, a simple method of highperformance multiplex microvalves chip fabrication was reported. In this method, a sandwich structure is established by inserting a polydimethylsiloxane (PDMS) membrane into two glasses, which is cheap and simple without any complex silicon-based device or soft lithography. Taking advantages of both the elasticity of the PDMS and the rigidity of glass, the microvalve chip showed good controls performance and had the ability of multiplex integration. Moreover, aided by a computer design program, this microvalves chip can be performed automatically, showing great potential to develop new highly integrated microfluidic devices. In addition, the fabricated multiplex microvalve chip is further successfully used for staining tumor cells automatically, improving the efficiency of cell identification process and reducing human errors. These results indicate this method opens up new avenues for multiplex microvalves fabrication and its biological application. Keywords Microfluidic chip . Microvalve . Tumor cell

1 Introduction

Shao-Li Hong, Man Tang and Zhengqi Chen contributed equally to this work. Electronic supplementary material The online version of this article (https://doi.org/10.1007/s10544-019-0434-5) contains supplementary material, which is available to authorized users. * Nangang Zhang [email protected] * Kan Liu [email protected] 1

School of Electronic and Electrical Engineering, Wuhan Textile University, Wuhan 430200, People’s Republic of China

2

Hubei Province Engineering Research Center for Intelligent Micro-nano Medical Equipment and Key Technologies, Wuhan 30200, People’s Republic of China

3

Hubei Engineering and Technology Research Center for Functional Fiber Fabrication and Testing, Wuhan 430200, People’s Republic of China

4

Hubei Key Laboratory of Digital Textile Equipment, Wuhan 430200, People’s Republic of China

5

School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu, Sichuan 610054, People’s Republic of China

Microfluidic technique offers great-perhaps revolutionary-new capabilities for the study of science and technology in future.(Whitesides 2006) Typically, the unique advantages of the microfluidic chip include rapid analysis(Chen et al. 2010), low sample and reagent consumption(Dittrich et al. 2006), high integration and versatility in design(Hagan et al. 2011; Thorsen et al. 2002), portability, disposability, and so on(Liu et al. 2007; Tanaka et al. 2007). Based on these, microfluidic technique has already applied in environment detection(Guo et al. 2015), medical diagnosis(Sa