Design and analysis of an optimized microfluidic channel for isolation of circulating tumor cells using deterministic la
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ORIGINAL ARTICLE
Design and analysis of an optimized microfluidic channel for isolation of circulating tumor cells using deterministic lateral displacement technique Rituraj Bhattacharjee1 · R. Kumar1 · Fenil Chetankumar Panwala1 · P. Mohamed Shakeel2 Received: 8 April 2020 / Accepted: 30 May 2020 © The Author(s) 2020
Abstract Circulating tumor cells (CTCs) are extremely scarce cells which cut off from a primary tumor and percolate into the circulation of blood flow and are, thus, critical for precise cancer detection and treatment. Deterministic lateral displacement (DLD) which exploits asymmetric splitting of laminar flow around the implanted microposts has displayed trustworthy capabilities in separating cells of varying sizes. In this research work, a microfluidic channel consisting of three symmetrically aligned inlets and outlets and embedded circular posts has been proposed which effectively separates the CTCs from lymphocytes utilizing the concept of DLD. Using a commercial software COMSOL Multiphysics 5.4, the design of the proposed microchannel has been simulated and analyzed considering an injected blood sample containing massive CTCs and slim WBCs of radii 13.5 µm and 6 µm, respectively. The proposed model of microchannel isolates the CTCs from WBCs at a comparatively higher sample mass flow rate of 4 × 10–6 kg/s and Reynolds number of 8.9 thereby operating efficiently at higher throughput, and offers excellent linearity in terms of velocity magnitude, pressure, shear rate and Reynolds number. The computational analysis of the proposed microchannel reveals that it can isolate CTCs from WBCs with better separation ratio, offers higher throughput, reduces possibilities of clogging and maintains better uniformity of pressure distribution and other flow parameters when compared with existing microchannel designs. The maximum separation ratio for CTCs and WBCs has been obtained as 84% and 96%, respectively. Keywords Circulating tumor cells · Deterministic lateral displacement · Microfluidics · COMSOL multiphysics · Bioparticles
Introduction The recent advancements in MEMS (microelectromechanical system) originating from silicon integrated circuit technology have drastically brought significant developments showcasing many technical breakthroughs, especially in the field of biotechnology. Over the recent years, an expeditious development of technology has been noticed in various domains of medical science out of which bio-particle * R. Kumar [email protected] 1
Department of Electronics and Instrumentation Engineering, National Institute of Technology Nagaland, Dimapur, Nagaland 797103, India
Faculty of Information and Communication Technology, Universiti Teknikal Malaysia Melaka (UTeM), Melaka, Malaysia
2
separation has captured prime attention as the research focus has shifted towards advancement in therapeutic methods for prevention of viral diseases. Many conventional analyses in biomedical industry mainly depend on the techniques and the operator’s experiences with the existing tra
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