High Sensitive Visual Protein Detection by Microfluidic Lateral Flow Assay with On-Stripe Multiple Concentration
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ORIGINAL
High Sensitive Visual Protein Detection by Microfluidic Lateral Flow Assay with On‑Stripe Multiple Concentration Shiyong Yu1 · Weiwei Sun1 · Pengjie Zhang1 · Yu Chen1 · Liben Yan1 · Lina Geng1 · Deng Yulin1 Received: 5 May 2020 / Revised: 21 June 2020 / Accepted: 3 July 2020 © Springer-Verlag GmbH Germany, part of Springer Nature 2020
Abstract Lateral flow assays (LAFs) especially integrated with a microfluidic chip, provides a simple, rapid, user-friendly, potable robust, and cost-effective technology for broad assays. However, this technology suffers from low sensitivity. In this paper, one kind of automatic roller of tap, which can be precisely controlled to replace sample tap was integrated into the microfluidic LAFs platform. And then, on-stripe repeated injection and concentration were realized with this simple mechanic unit. The minimum detection concentration for human chorionic gonadotropin (HCG) was 1.26 ng/mL, comparable with literature using complex enzyme/chemical reaction-based signal amplification. The linear relationship between the signal intensity and enrichment times reflected the good reproducibility of the novel device. At the same time, the good linear relationship between the predicted accumulation quantity of HCG and the gray value of bands is very meaningful for quantitative detection. Consequently, this novel universal approach shows great potential in the rapid trace analysis and broaden the application of LAFs with its attractive characteristics. Keywords Lateral flow · Microfluidic chip · Sensitivity · HCG
Introduction Being rapid, inexpensive, easy-to-manufacture and userfriendly, lateral flow assays (LFAs) have usually been the first technology to be considered in a range of fields, including medical diagnostics, bedside analysis, food safety, and environmental safety for biochemical analytes, such as proteins, glycolipids, lipids, and nucleic acids [1–5]. Microfluidics technology brings great potential by providing integration, high-throughput, fast analysis time, portability, and small reagent volume. Taking advantage of the microfluidics, there have been a lot of efforts to integrated lateral flow assays (LAFs) with the microfluidic device to realize integrated assay platform with multifunction, for example, an integrated rotary microfluidic system with DNA extraction, loop-mediated isothermal amplification, and lateral flow strip based detection for point-of-care pathogen diagnostics [6]. At the same time, various microfluidic elements * Shiyong Yu [email protected] 1
School of Life Science and Technology, Beijing Institute of Technology, 5 South3 Zhongguancun Street, Haidian, Beijing, China
such as hydraulic resistors [7, 8] valves [9], and advanced features for liquid flow control [10] were also introduced into LAFs. However, due to the limit in the space of LFAs and microfluidic chip, the outstanding issues on detection sensitivity remain unsettled, which is an urgent problem in their practical application. Especially in the case of real sample analysis, s
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