High Throughput Screening Methods and Protocols, Second Edition
In the years since the release of the popular first edition, the field of High Throughput Screening (HTS) has evolved considerably, from a small niche area of study to a major, essential scientific technique. In High Throughput Screening: Methods and Prot
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M O L E C U L A R B I O L O G Y TM
Series Editor John M. Walker School of Life Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK
For other titles published in this series, go to www.springer.com/series/7651
High Throughput Screening Methods and Protocols, Second Edition
Edited by
William P. Janzen University of North Carolina, Chapel Hill, NC, USA and
Paul Bernasconi BASF Corporation, Research Triangle Park, NC, USA
Editors William P. Janzen UNC Eshelman School of Pharmacy Ctr Integrative Chemical Bio & Drug Disc Division of Medicinal Chem & Natural Products University of North Carolina Chapel Hill NC 27599-763 USA [email protected]
Paul Bernasconi BASF Corporation Research Triangle Park NC 27709-3528 USA [email protected]
ISBN 1064-3745 e-ISBN 1940-6029 ISBN 978-1-60327-257-5 e-ISBN 978-1-60327-258-2 DOI 10.1007/978-1-60327-258-2 Springer Dordrecht Heidelberg London New York Library of Congress Control Number: 2009929642 # Humana Press, a part of Springer ScienceþBusiness Media, LLC 2002, 2009 All rights reserved. This work may not be translated or copied in whole or in part without the written permission of the publisher (Humana Press, c/o Springer Science+Business Media, LLC, 233 Spring Street, New York, NY 10013, USA), except for brief excerpts in connection with reviews or scholarly analysis. Use in connection with any form of information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed is forbidden. The use in this publication of trade names, trademarks, service marks, and similar terms, even if they are not identified as such, is not to be taken as an expression of opinion as to whether or not they are subject to proprietary rights. While the advice and information in this book are believed to be true and accurate at the date of going to press, neither the authors nor the editors nor the publisher can accept any legal responsibility for any errors or omissions that may be made. The publisher makes no warranty, express or implied, with respect to the material contained herein. Printed on acid-free paper Springer is part of Springer ScienceþBusiness Media (www.springer.com)
Preface In the 6 years since the first edition of this book, the field of high-throughput screening (HTS) has evolved considerably. In 2004, the Society for Biomolecular Screening (SBS) celebrated its 10th anniversary. The event and its timing were significant because SBS is the world’s largest association of scientists, engineers and technologists associated with HTS. While the creation of SBS did not mark the birth of HTS by any means, its foundation in 1994 helped HTS find a common voice. It provided a discussion forum and a means to define and enforce standards. In 2006, SBS became the Society for Biomolecular Sciences, underlining the expansion of the members’ interests beyond screening. Like any new technology, HTS went through growth stages. During the initial hype phase of the 1980s and 1990s,
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