Lentivirus Gene Engineering Protocols Second Edition
Since the publication of the first edition, lentivirus vector-based technologies, through in vitro and in vivo gene transfer in eukaryotic animal cells, continue to offer the most promising opportunities for curing genetic disorders, as well as cancer and
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Molecular Biology™
Series Editor John M. Walker School of Life Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK
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Lentivirus Gene Engineering Protocols Second Edition
Edited by
Maurizio Federico Division of Pathogenesis of Retroviruses, National AIDS Center, Istituto Superiore di Sanità, Rome, Italy
Editor Maurizio Federico, Ph.D. Division of Pathogenesis of Retroviruses National AIDS Center Istituto Superiore di Sanità Rome Italy [email protected]
ISSN 1064-3745 e-ISSN 1940-6029 ISBN 978-1-60761-532-3 e-ISBN 978-1-60761-533-0 DOI 10.1007/978-1-60761-533-0 Springer New York Dordrecht Heidelberg London Library of Congress Control Number: 2009942262 © Humana Press, a part of Springer Science+Business Media, LLC 2010 All rights reserved. This work may not be translated or copied in whole or in part without the written permission of the publisher (Humana Press, c/o Springer Science+Business Media, LLC, 233 Spring Street, New York, NY 10013, USA), except for brief excerpts in connection with reviews or scholarly analysis. Use in connection with any form of information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed is forbidden. The use in this publication of trade names, trademarks, service marks, and similar terms, even if they are not identified as such, is not to be taken as an expression of opinion as to whether or not they are subject to proprietary rights. While the advice and information in this book are believed to be true and accurate at the date of going to press, neither the authors nor the editors nor the publisher can accept any legal responsibility for any errors or omissions that may be made. The publisher makes no warranty, express or implied, with respect to the material contained herein. Cover illustration: Derived from Chapter 9, figure 1A. Printed on acid-free paper Humana Press is part of Springer Science+Business Media (www.springer.com)
Dedicated to my wife, Margherita, and to the memory of my mother, Ottavia. As a token of my gratitude for their love.
Preface This second edition of the Lentivirus Gene Engineering Protocols book does not simply update several aspects of the techniques detailed in the previous edition published in 2003. Rather, it mirrors the spectacular applicative progress which has occurred in the field of lentiviral vector (LV)-based gene engineering over the past few years. On the other hand, considering that many LV-related techniques (e.g., LV titration and challenge, detection and selection of transduced cells, detection of replication-competent viral particles) did not meet relevant modifications over the years, readers interested in these and other matters not included in the present book can still conveniently refer to the previous edition. Conversely, an almost new set of topics is included in this second edition. The only exception is the contribution
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