Hollow Prussian Blue nanocubes as peroxidase mimetic and enzyme carriers for colorimetric determination of ethanol
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ORIGINAL PAPER
Hollow Prussian Blue nanocubes as peroxidase mimetic and enzyme carriers for colorimetric determination of ethanol Shuangling Wang 1 & Han Yan 1 & Yalin Wang 1 & Na Wang 1 & Yulong Lin 1 & Meng Li 1 Received: 4 May 2019 / Accepted: 14 September 2019 # Springer-Verlag GmbH Austria, part of Springer Nature 2019
Abstract The peroxidase-like activity of hollow Prussian Blue nanocubes (hPBNCs) is used, in combination with the enzyme alcohol oxidase (AOx), in a colorimetric ethanol assay. Different from other nanozymes, the large cavity structure of the hPBNCs provides a larger surface and more binding sites for AOx to be bound on their surface or in the pores. This extremely enhances the sensitivity of the assay system. In the presence of ethanol, AOx is capable of catalyzing the oxidation of alcohols to aldehydes, accompanied by the generation of hydrogen peroxide (H2O2). The hPBNCs act as peroxidase mimics and then can catalyze the oxidation of 3,3′5,5′-tetramethylbenzidine (TMB) by H2O2, resulting in a color change of the solution from colorless to blue with a strong absorption at 652 nm. The lower detection limit for ethanol is 1.41 μg∙mL−1. Due to the high catalytic activity of hPBNCs in weakly acidic and neutral solutions, the system was successfully applied to the determination of ethanol in mice blood. This is critically important for studying the alcohol consumption and monitoring the ethanol toxicokinetics. Keywords Nanozymes . Nanocomposites . Cavity structure . Catalytic activity . Alcohol oxidase . Hydrogen peroxide . Chromogenic substrate . Blood analysis . Ethanol toxicokinetics . Optical sensor
Introduction Alcohol over-consumption has been reported to lead to variety of diseases, disorders and many types of injury, which would result in approximately 2.5 million deaths each year [1–3]. Owing to the significance of its toxicokinetics, the accurate and sensitive determination of alcohol level in vivo is of particular interest, especially its concentration dynamics. To measure alcohol consumption, different methods for detection of the concentration of alcohol in blood, urine, breath, sweat, saliva or other body fluids have been demonstrated [4–7], in which blood alcohol concentration is recognized as the most widely used indicator of alcohol
Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00604-019-3826-6) contains supplementary material, which is available to authorized users. * Yulong Lin [email protected] * Meng Li [email protected] 1
School of Pharmaceutical Sciences, Hebei Medical University, Zhongshan East Road 361, Shijiazhuang 050017, People’s Republic of China
intoxication. Thus, sensitive detection and quantification of alcohol in blood has important significance for diagnostic and therapeutic implications. For this purpose, a series of methods and techniques for accurate and precise determination of alcohol level in blood have been employed, including gas chromatography [8], liquid chromatography [9], spectroscopy [10–14],
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