Silver nanoparticles conjugated MnFe-based Prussian blue analogue for voltammetric and impedimetric bioaptasensing of am
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ORIGINAL PAPER
Silver nanoparticles conjugated MnFe-based Prussian blue analogue for voltammetric and impedimetric bioaptasensing of amifostine (ethyol) Saad A. Alkahtani 1 Received: 7 June 2020 / Accepted: 13 September 2020 / Published online: 25 September 2020 # Springer-Verlag GmbH Austria, part of Springer Nature 2020
Abstract A novel bioaptasensing-based electrochemical method for determination of amifostine (AMF) is proposed. The electrochemical aptasensor is based on modification of a glassy carbon electrode with a nanocomposite consisting of silver nanoparticles @ MnFe Prussian blue analogue nanospheres (AgNPs@MnFePBA NS), followed by immobilization of aptamer via Ag-N bonds (aptamer/AgNPs@MnFePBA NS/GCE). Experimental parameters including pH, incubation time, and aptamer concentrations were optimized. Electrochemical impedance spectroscopy (EIS) and differential pulse voltammetric (DPV) techniques were utilized to quantify AMF. The anodic peak current (ΔIpa) and charge transfer resistance (ΔRct) differences increase in the presence of AMF. Under the optimal conditions, using the redox probe, the electrochemical aptasensor exhibited linear ranges of 0.34–45 nmol L−1 and 0.69–45 nmol L−1 with LODs of 0.11 nmol L−1 and 0.23 nmol L−1 for EIS and DPV, respectively. The aptasensor was used to determine AMF in human plasma and in the presence of interfering species with recoveries and RSDs in the range 97.8–103.2% and 2.2–4.2%, respectively. Keywords Amifostine . MnFe Prussian blueanalogue . Nanosphere . Differentialpulse voltammetry . Electrochemicalimpedance spectroscopy
Introduction Amifostine (AMF), a synthetic aminothiol compound, is approved by USFDA for the use as a cytoprotective agent in chemo- and radiotherapies involving DNA-binding chemotherapeutic drugs. AMF is converted in vivo by alkaline phosphatase present in normal tissue not in the tumor tissue, to its thiol derivative (AMF thiol) which is a protective compound [1, 2]. AMF thiol is able to remove free radicals and active metabolites of anti-cancer agents. Moreover, AMF thiol can hydrate electrons that damage DNA via SH group [3]. In comparison with AMF, AMF thiol only provides protection to non-central nervous Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00604-020-04557-4) contains supplementary material, which is available to authorized users. * Saad A. Alkahtani [email protected] 1
Department of Clinical Pharmacy, College of Pharmacy, Najran University, Najran, Kingdom of Saudi Arabia
system (CNS) tissues since it is not able to cross the blood–brain barrier [4]. AMF has been determined by fluorometry [5–7], capillary electrophoresis [8], and HPLC [9, 10]. Disadvantages of most of these methods are time-consuming, not reproducible, high cost of instrumentation, and high treatment cost. In contrast, electroanalytical techniques stand out as a promising and an effective technique owing to simplicity, wide-dynamic range, low detection limit, good selectivity, fast response, and no ne
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