Host reaction of tomato varieties and applicability of Sm -linked DNA markers to Stemphylium lycopersici
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Host reaction of tomato varieties and applicability of Sm-linked DNA markers to Stemphylium lycopersici Jiyeon Park & Seungahn Kwon & Girim Park & Siyoung Lee & Byoung Il Je & Younghoon Park
Received: 5 August 2020 / Accepted: 5 November 2020 # Springer Nature B.V. 2020
Abstract Gray leaf spot (GLS) disease, caused by the Stemphylium (Sm) fungus, affects a wide range of horticultural crops including tomato. In this study, the South Korean Sm isolate (PNU-SM01) was identified, and the applicability of Sm-resistance gene (Sm)-linked DNA markers in marker-assisted selection was validated. ITS/gpd sequencing and morphological observation identified the isolate as S. lycopersici. A GLS disease assay was conducted on 80 tomato varieties, including commercial F1 hybrids and elite inbred lines. The reference line ‘Motelle’ was highly resistant (R, DSI = 4.0), whereas the ‘Moneymaker’ line was susceptible (S) and showed severe disease symptoms (DSI = 79.3). Among 30 commercial F1 hybrids, five were scored as S by DSI; whereas the rest, including all 17 hybrids claimed as Sm-resistant by the supplier, were R or moderately resistant (MR). Among 48 inbred lines, four were scored as S and 44, including five Lycopersicon accessions reported as R, were R or MR. Three known Sm-linked DNA markers (CT55, D5, and Sm-InDel) were genotyped in all 80 cultivars; the highest match (92.5%) to the GLS disease assay was observed for Sm-InDel. We converted the recessive marker CT55 into a codominant marker (CT55-Co) and developed dCAPS (Sm-SNP) and KASP (Sm-KASP) markers from a previously identified Sm-linked SNP. Sm-SNP and Sm-KASP showed Jiyeon Park and Seungahn Kwon contributed equally to this work. J. Park : S. Kwon : G. Park : S. Lee : B. Je : Y. Park (*) Department of Horticultural Bioscience, Pusan National University, Miryang 50463, South Korea e-mail: [email protected]
a similar level of phenotype to marker genotype association as Sm-InDel, whereas CT55-Co displayed a high level of mismatch. To screen for the source of Sm resistance, 138 tomato accessions were genotyped with Sm-InDel and Sm-SNP: 15 were homozygous for the resistance allele of both markers. In this study, resistance to the Korean isolate of S. lycopersici in commercial F1 hybrids and breeding sources was characterized to reveal which DNA markers can be effectively applied for marker-assisted selection of Sm-resistance. Keywords Gray leaf spot disease . Marker-assisted selection . Single nucleotide polymorphism . Solanum lycopersicum . Stemphylium resistance Abbreviations CAPS Cleaved amplified polymorphic sequence dCAPS derived CAPS DSI Disease severity index GLS Gray leaf spot ITS Internal transcribed spacer KASP Kompetitive allele-specific PCR MAS Marker-assisted selection NCBI National Center for Biotechnology Information PGBRC Plant Genetics and Breeding Research Center RFLP Restriction fragment length polymorphism SCAR Sequence characterized amplified region SGN Sol Genomics Network Sm Stemphylium Sm Sm-resistance gene
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SNP LA TGRC VIGS
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