Cancer Cell Culture Methods and Protocols
With many recent advances, cancer cell culture research is more important than ever before. This timely edition of Cancer Cell Culture: Methods and Protocols covers the basic concepts of cancer cell biology and culture while expanding upon the recent shif
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Molecular Biology™
Series Editor John M. Walker School of Life Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK
For further volumes: www.springer.com/series/7651
Cancer Cell Culture Methods and Protocols
Second Edition Edited by
Ian A. Cree Translational Oncology Research Centre, Queen Alexandra Hospital, Portsmouth, UK
Editor Ian A. Cree, M.B. Ch.B., Ph.D., F.R.C.Path. Translational Oncology Research Centre Queen Alexandra Hospital Portsmouth UK [email protected]
ISSN 1064-3745 e-ISSN 1940-6029 ISBN 978-1-61779-079-9 e-ISBN 978-1-61779-080-5 DOI 10.1007/978-1-61779-080-5 Springer New York Dordrecht Heidelberg London Library of Congress Control Number: 2011926687 © Springer Science+Business Media, LLC 2011 All rights reserved. This work may not be translated or copied in whole or in part without the written permission of the publisher (Humana Press, c/o Springer Science+Business Media, LLC, 233 Spring Street, New York, NY 10013, USA), except for brief excerpts in connection with reviews or scholarly analysis. Use in connection with any form of information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed is forbidden. The use in this publication of trade names, trademarks, service marks, and similar terms, even if they are not identified as such, is not to be taken as an expression of opinion as to whether or not they are subject to proprietary rights. While the advice and information in this book are believed to be true and accurate at the date of going to press, neither the authors nor the editors nor the publisher can accept any legal responsibility for any errors or omissions that may be made. The publisher makes no warranty, express or implied, with respect to the material contained herein. Printed on acid-free paper Humana Press is part of Springer Science+Business Media (www.springer.com)
Preface Cancer cell culture remains as important today as it was when the first edition of Cancer Cell Culture: Methods and Protocols was published 6 years ago. However, the emphasis of research using cell culture methods is shifting towards the use of primary cells rather than the generation of new cell lines, and the chapters in this edition reflect this. As before, the basic concepts of cancer cell biology and culture are covered in the first few chapters, with an emphasis on safe working practice for both cells and laboratory researchers. These chapters contain the information critical to success – only by good practice and quality control will the results of cancer cell culture improve. I am grateful to several authors from the last edition for updating some of their chapters to reflect improvements, particularly the rapid improvement in real-time PCR technology. In a change to the last edition, which is still available, I have included a series of chapters covering a series of different assay methods which allow the action of drugs and toxins on cells to be measured. Many of thes
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