Human Umbilical Cord Blood Mesenchymal Stem Cell Conditioned Medium (hMSC-CM) Improves Antioxidant Status in Carbon Tetr
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RESEARCH PAPER
Human Umbilical Cord Blood Mesenchymal Stem Cell Conditioned Medium (hMSC-CM) Improves Antioxidant Status in Carbon Tetrachloride-Induced Oxidative Damage in Rat Mahdi Bahmani1 • Nasrin Ziamajidi1 • Mohammad Hashemnia2 • Roghayeh Abbasalipourkabir1 Aref Salehzadeh3
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Received: 4 February 2020 / Accepted: 16 July 2020 Ó Shiraz University 2020
Abstract Carbon tetrachloride (CCl4) as environmental toxin induces tissue damage through oxidative stress. In the present study, the effect of treatment with Human Umbilical Cord Blood Mesenchymal Stem Cell Conditioned Medium (hMSC-CM) was evaluated on improvement of chronic injectable CCl4-induced oxidative stress. Fifty-four male rats were divided into nine groups: N (PBS-treated group), O8 and O12 (Olive oil-treated group for 8 and 12 weeks, respectively), D8 and D12 (hMSC-CM-treated group for 4 and 8 weeks, respectively), C8 and C12 (CCl4-treated group for 8 and 12 weeks, respectively), T8 (CCl4- and hMSC-treated group resembled C8 and D8, respectively), T12 (CCl4- and hMSC-treated group resembled C12 and D12, respectively). Then, oxidative stress markers were evaluated in the liver tissue. To evaluate histopathological lesions, Masson’s trichrome and H&E stainings were performed. Although treatment with hMSC-CM resulted in a significant increase in the total antioxidant capacity (TAC), total thiol groups (TTG), and a significant decrease in the total oxidant status (TOS) and MDA (malondialdehyde) compared to CCL4 group, only MDA activity returned to normal base which observed in the control group. Treatment with hMSC-CM increased superoxide dismutase (SOD), glutathione peroxidase (GPX), and catalase (CAT) activity compared to groups received only CCl4. Also treatment with hMSC-CM improved CCl4-induced histopathological lesions. Results of the present study demonstrated that the hMSCCM as a potential antioxidant could be an effective therapeutic agent against CCl4-induced liver injury. Keywords CCl4 Oxidative Stress hMSC-CM Rat Liver
& Roghayeh Abbasalipourkabir [email protected] Mahdi Bahmani [email protected] Nasrin Ziamajidi [email protected] Mohammad Hashemnia [email protected] Aref Salehzadeh [email protected] 1
Department of Biochemistry, School of Medicine, Hamadan University of Medical Sciences, Hamadan 65178, Iran
2
Departments of Pathobiology, Veterinary Medicine Faculty, Razi University, Kermanshah, Iran
3
Department of Entomology, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran
1 Introduction Occupational and environmental hepatotoxicity is increasingly growing around the world (Tolman and Dalpiaz 2013). Environment and occupation-related liver diseases occur due to exposure to chemical compounds and environmental contaminants (Shah et al. 2017; Yu et al. 2002). The liver plays a critical role in the metabolism of xenobiotic compounds which consequently cause toxicity in the liver (Ravan et al. 2017). Carbon tetrachloride (CCl4) is a co
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