Human Umbilical Cord Blood Cells Alter Blood and Spleen Cell Populations After Stroke
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ORIGINAL ARTICLE
Human Umbilical Cord Blood Cells Alter Blood and Spleen Cell Populations After Stroke J. E. Golden & M. Shahaduzzaman & A. Wabnitz & S. Green & T. A. Womble & P. R. Sanberg & K. R. Pennypacker & A. E. Willing
Received: 5 June 2012 / Revised: 13 August 2012 / Accepted: 14 August 2012 / Published online: 4 September 2012 # Springer Science+Business Media, LLC 2012
Abstract The human umbilical cord blood (HUCB) mononuclear cell (MNC) fraction is a mixed population of cells that induces functional repair in rodent models of stroke when injected intravenously (i.v.). The transplanted cells are found in the infarcted hemisphere and the spleen. The goal of this project was to determine the nature of the interaction between the HUCB MNCs and splenic immune cells. Male Sprague Dawley rats underwent permanent middle cerebral artery occlusion (MCAO) and received i.v. injection of either vehicle (MCAO only), HUCB MNCs, or MNCs depleted of CD14+ monocytes, CD133+ stem cells, or CD19+ B cells 48 h poststroke. At 72 h postMCAO, the animals were euthanized, and the spleens and blood MNCs were harvested for flow cytometry and mitogen proliferation assays. All HUCB cell preparations decreased the percentage of T cells in the spleen and monocytes in the blood (p
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