Hydrogen peroxide sensing in body fluids and tumor cells via in situ produced redox couples on two-dimensional holey CuC
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ORIGINAL PAPER
Hydrogen peroxide sensing in body fluids and tumor cells via in situ produced redox couples on two-dimensional holey CuCo2O4 nanosheets Jingwen Xie 1 & Dan Cheng 1 & Zile Zhou 1 & Xiao Pang 1 & Meiling Liu 1 Xiaoying Liu 1,2 & Shouzhuo Yao 1
&
Peng Yin 1 & Youyu Zhang 1 & Haitao Li 1 &
Received: 17 January 2020 / Accepted: 13 June 2020 # Springer-Verlag GmbH Austria, part of Springer Nature 2020
Abstract A novel nanomaterial of two-dimensional holey CuCo2O4 (2D HCCO) nanosheets was synthesized via a general templatedirected method and employed for the first time to construct an effective electrochemical platform for H2O2 sensing with the combination of cerium oxide (CeO2). During the electrocatalytic reduction of H2O2, the synergetic catalysis of CeO2/HCCO/ MWCNTs/GCE owing to the naturally holey frameworks and the mediator of CeO2 results in the ultra-sensitive detection of H2O2. The current was greatly enhanced owing to the unique holey structure that can minimize the charge transfer distance and provide more active sites to boost the signals, and the dual oxidation state of Ce3+/Ce4+ on the surface of 2D HCCO nanosheets can promote the in situ production of Cu2+/Cu+ and Cu+/Cu and further amplify the detection signal. The CeO2/HCCO/ MWCNTs/GCE showed a wide linear range from 1 μM to 7.31 mM using chronoamperometry at the potential of − 0.25 V and a relatively low detection limit of 0.16 μM in physiological environment, which was also utilized for tracking the trace H2O2 released from Hela cells. This study shows great promise for the emerging application of holey HCCO-based biosensors in bioanalysis and early cancer diagnosis. Keywords Synergetic catalysis . Holey mixed transition metal oxide nanosheets . Redox mediator . Cerium oxide (CeO2) . Hydrogen peroxide free-enzymatic sensor . Tumor cells
Introduction Hydrogen peroxide (H2O2), a typical marker of tumor microenvironment as well as the anti-tumoral immune response, plays an essential role in denoting cancer development [1, Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00604-020-04389-2) contains supplementary material, which is available to authorized users. * Meiling Liu [email protected] * Xiaoying Liu [email protected] 1
Key Laboratory of Chemical Biology & Traditional Chinese Medicine Research (Ministry of Education, China), College of Chemistry and Chemical Engineering, Hunan Normal University, Changsha 410081, People’s Republic of China
2
School of Chemistry and Materials Science, Hunan Agricultural University, Changsha 410128, People’s Republic of China
2]. Moderate levels of H2O2 act as a second messenger involved in cell signal transduction to control cell proliferation, differentiation, and survival, and participate in the regulation of gene transcription [3, 4]. Nevertheless, an increase in H2O2 is produced when normal cell carcinogenesis occurs, which destructs the intracellular balance and triggers oxidative stress leading to cell death, tissue damage, and even cardi
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