Identification and validation of genetic loci for tiller angle in bread wheat
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ORIGINAL ARTICLE
Identification and validation of genetic loci for tiller angle in bread wheat Dehui Zhao1,2 · Li Yang1 · Kaijie Xu3 · Shuanghe Cao1 · Yubing Tian1 · Jun Yan3 · Zhonghu He1,4 · Xianchun Xia1 · Xiyue Song2 · Yong Zhang1 Received: 20 March 2020 / Accepted: 8 July 2020 © Springer-Verlag GmbH Germany, part of Springer Nature 2020
Abstract Key message Two major QTL for tiller angle were identified on chromosomes 1AL and 5DL, and TaTAC-D1 might be the candidate gene for QTA.caas-5DL. Abstract An ideal plant architecture is important for achieving high grain yield in crops. Tiller angle (TA) is an important factor influencing yield. In the present study, 266 recombinant inbred lines (RILs) derived from a cross between Zhongmai 871 (ZM871) and its sister line Zhongmai 895 (ZM895) was used to map TA by extreme pool-genotyping and inclusive composite interval mapping (ICIM). Two quantitative trait loci (QTL) on chromosomes 1AL and 5DL were identified with reduced tiller angle alleles contributed by ZM895. QTA.caas-1AL was detected in six environments, explaining 5.4–11.2% of the phenotypic variances. The major stable QTL, QTA.caas-5DL, was identified in all eight environments, accounting for 13.8–24.8% of the phenotypic variances. The two QTL were further validated using B C1F4 populations derived from backcrosses ZM871/ZM895//ZM871 (121 lines) and ZM871/ZM895//ZM895 (175 lines). Gene TraesCS5D02G322600, located in the 5DL QTL and designated TaTAC-D1, had a SNP in the third exon with ‘A’ and ‘G’ in ZM871 and ZM895, respectively, resulting in a Thr169Ala amino acid change. A KASP marker based on this SNP was validated in two sets of germplasm, providing further evidence for the significant effects of TaTAC-D1 on TA. Thus extreme pool-genotyping can be employed to detect QTL for plant architecture traits and KASP markers tightly linked with the QTL can be used in wheat breeding programs targeting improved plant architecture.
Introduction Communicated by Jorge Dubcovsky. Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00122-020-03653-6) contains supplementary material, which is available to authorized users. * Xiyue Song [email protected] * Yong Zhang [email protected] 1
Institute of Crop Sciences, National Wheat Improvement Centre, Chinese Academy of Agricultural Sciences (CAAS), 12 Zhongguancun South Street, Beijing 100081, China
2
College of Agronomy, Northwest A&F University, Yangling 712100, Shaanxi province, China
3
Institute of Cotton Research, CAAS, 38 Huanghe Dadao, Anyang 455000, Henan province, China
4
CIMMYT-China Office, c/o CAAS, 12 Zhongguancun South Street, Beijing 100081, China
Plant architecture determined mainly by plant height, tiller number and angle, and panicle morphology affects photosynthetic efficiency, stress response, grain yield, and grain quality in rice (Wang and Li 2008). Wheat plants with desirable architecture are able to produce higher grain yields, as was the case in the “Green Revolution” (GR
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