Identification, characterisation and expression analysis of MADS-box genes in sweetpotato wild relative Ipomoea trifida
- PDF / 2,121,924 Bytes
- 13 Pages / 595.276 x 790.866 pts Page_size
- 35 Downloads / 225 Views
ORIGINAL ARTICLE
Identification, characterisation and expression analysis of MADS‑box genes in sweetpotato wild relative Ipomoea trifida Panpan Zhu1,2 · Tingting Dong1 · Tao Xu1 · Hunseung Kang2 Received: 18 July 2019 / Revised: 8 October 2020 / Accepted: 15 October 2020 © Franciszek Górski Institute of Plant Physiology, Polish Academy of Sciences, Kraków 2020
Abstract MADS-box transcription factors (TFs) participate in various biological processes, including stress response, plant growth and development. The storage root of sweetpotato (Ipomoea batatas) is an important staple food crop in Asian and African areas, and leafy sweetpotato is a popular and healthy vegetable in China. However, the function of the MADS genes remains largely unknown in sweetpotato and the other Ipomoea species. In this study, we carried out a genome-wide gene family analysis of MADS in Ipomoea trifida which is currently considered as the most potential ancestor of sweetpotato. A total of 37 ItfMADS genes that encode 42 proteins were identified. The evolutionary relationship was analysed for MADS proteins in 37 species. According to the tree topology of phylogeny, the ItfMADS genes are classified into Mα, Mβ, Mγ, MIKCC and MIKC* groups. Gene structure analysis revealed that the majority of ItfMADS in the same clade displayed similar exon–intron distribution. Furthermore, the expression patterns of ItfMADS genes were investigated through RNA-seq data and confirmed by qRT-PCR. The expression of ItfMADS genes varied among different tissues, and induced or repressed under different abiotic stresses, thereby suggesting their potential functions under abiotic stresses. Our findings lay a solid foundation for further elucidating the ItfMADS function and promoting sweetpotato molecular breeding. Keywords Ipomoea trifida · Sweetpotato · MADS-box · Phylogenetic analysis · Gene expression
Introduction
Communicated by T. Xu. Panpan Zhu and Tingting Dong contributed equally to this work. Electronic supplementary material The online version of this article (https://doi.org/10.1007/s11738-020-03153-6) contains supplementary material, which is available to authorized users. * Tao Xu [email protected] * Hunseung Kang [email protected] 1
Jiangsu Key Laboratory of Phylogenomics and Comparative Genomics, School of Life Sciences, Jiangsu Normal University, Xuzhou 221116, Jiangsu Province, China
Department of Applied Biology, College of Agriculture and Life Sciences, Chonnam National University, Gwangju 61186, South Korea
2
MADS transcription factors (TFs) contain a highly conserved MADS domain which is a DNA-binding domain of 60 amino acids long (Messenguy and Dubois 2003; Riechmann and Meyerowitz 1997). MADS-box genes are crucial for plant growth and development, especially in the genesis and formation of flower and reproductive organ (Causier et al. 2002). Based on the evolutionary lineages, MADS TFs in plants are categorised into type I or II. Type II genes contain three additional conserved domains apart from the MADS domains, namely, th
Data Loading...
