Genome-wide identification and expression analyses of nitrate transporter family genes in wild soybean ( Glycine soja )
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PLANT GENETICS • ORIGINAL PAPER
Genome-wide identification and expression analyses of nitrate transporter family genes in wild soybean (Glycine soja) Hongguang You 1 & Yuanming Liu 1 & Thuy Nguyen Minh 1 & Haoran Lu 1 & Pengmin Zhang 1 & Wenfeng Li 1 & Jialei Xiao 1 & Xiaodong Ding 1 & Qiang Li 1 Received: 10 March 2020 / Revised: 25 June 2020 / Accepted: 20 July 2020 # Institute of Plant Genetics, Polish Academy of Sciences, Poznan 2020
Abstract Nitrate transporters (NRTs) are important channel proteins facilitating cross-membrane movement of small molecules like NO3− which is a critical nutrient for all life. However, the classification and evolution of nitrate transporters in the legume plants are still elusive. In this study, we surveyed the wild soybean (G. soja) genomic databases and identified 120 GsNRT1 and 5 GsNRT2 encoding genes. Phylogenetic analyses show that GsNRT1 subfamily is consisted of eight clades (NPF1 to NPF8), while GsNRT2 subfamily has only one clade. Gene chromosomal location and evolutionary historic analyses indicate that GsNRT genes are unevenly distributed on 19 out of 20 G. soja chromosomes and segmental duplications may take a major part in the expansion of GsNRT family. Investigations of gene structure and protein motif compositions suggest that GsNRT family members are highly conserved in structures of both gene and protein levels. In addition, we analyzed the spatial expression patterns of representative GsNRT genes and their responses to exogenous nitrogen and carbon supplies and different abiotic stresses. The qRT-PCR data indicated that 16 selected GsNRT genes showed various expression levels in the roots, stems, leaves, and pods of young G. soja plants, and these genes were regulated by not only nitrogen and carbohydrate nutrients but also NaCl, NaHCO3, abscisic acid (ABA), and salicylic acid (SA). These results suggest that GsNRT genes may be involved in the regulation of plant growth, development, and adaptation to environmental stresses, and the study will shed light on functional dissection of plant nitrate transporter proteins in the future. Keywords Nitrate transporter . Wild soybean . Expression pattern . Phylogenetic analyses . Genetics
Communicated by: Izabela Pawłowicz Electronic supplementary material The online version of this article (https://doi.org/10.1007/s13353-020-00571-7) contains supplementary material, which is available to authorized users. * Qiang Li [email protected]
Pengmin Zhang [email protected]
Hongguang You [email protected]
Wenfeng Li [email protected]
Yuanming Liu [email protected]
Jialei Xiao [email protected]
Thuy Nguyen Minh [email protected]
Xiaodong Ding [email protected]
Haoran Lu [email protected]
1
Key Laboratory of Agricultural Biological Functional Genes, Northeast Agricultural University, Harbin 150030, China
J Appl Genetics
Abbreviations CDS Coding sequence CHL1 Chlorate resistant mutant 1 NRT Nitrate transporter MFS Major facilitator superfamily HMM Hidden Markov model TD Tandem duplicated SD Segmental duplicated
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