Identification, gene expression and genetic polymorphism of zinc finger A20/AN1 stress-associated genes, HvSAP , in salt
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RESEARCH
Open Access
Identification, gene expression and genetic polymorphism of zinc finger A20/AN1 stress-associated genes, HvSAP, in salt stressed barley from Kazakhstan Akmaral Baidyussen1†, Maryam Aldammas2†, Akhylbek Kurishbayev1, Malika Myrzabaeva1, Askar Zhubatkanov1, Grigory Sereda3, Raisa Porkhun3, Sergey Sereda3, Satyvaldy Jatayev1*, Peter Langridge4, Carly Schramm2, Colin L. D. Jenkins2, Kathleen L. Soole2 and Yuri Shavrukov2* From Fifth International Scientific Conference “Plant Genetics, Genomics, Bioinformatics, and Biotechnology” (PlantGen2019) Novosibirsk, Russia. 24-29 June 2019
Abstract Background: A family of genes designated as the Zinc finger A20/AN1 Transcription factors encoding stressassociated proteins (SAP) are well described in Arabidopsis and rice, and include 14 AtSAP and 18 OsSAP genes that are associated with variable tolerances to multiple abiotic stresses. The SAP gene family displays a great diversity in its structure and across different plant species. The aim of this study was to identify all HvSAP genes in barley (Hordeum vulgare L.), to analyse the expression of selected genes in response to salinity in barley leaves and develop SNP marker for HvSAP12 to evaluate the association between genotypes of barley plants and their grain yield in field trials. Results: In our study, 17 HvSAP genes were identified in barley, which were strongly homologous to rice genes. Five genes, HvSAP5, HvSAP6, HvSAP11, HvSAP12 and HvSAP15, were found to be highly expressed in leaves of barley plants in response to salt stress in hydroponics compared to controls, using both semi-quantitative RT-PCR and qPCR analyses. The Amplifluor-like SNP marker KATU-B30 was developed and used for HvSAP12 genotyping. A strong association (R2 = 0.85) was found between KATU-B30 and grain yield production per plant of 50 F3 breeding lines originating from the cross Granal × Baisheshek in field trials with drought and low to moderate salinity in Northern and Central Kazakhstan. (Continued on next page)
* Correspondence: [email protected]; [email protected] † Akmaral Baidyussen and Maryam Aldammas contributed equally to this work. 1 Faculty of Agronomy, S. Seifullin Kazakh AgroTechnical University, Nur-Sultan, Kazakhstan 2 College of Science and Engineering, Biological Sciences, Flinders University, Adelaide, SA, Australia Full list of author information is available at the end of the article © The Author(s). 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons lice
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