Zinc Finger Proteins Methods and Protocols
This detailed volume explores the notable progress in the field of zinc finger proteins (ZFP) study through widely used methods and protocols involving their biological functions and applications. Beginning with a section on the basic biology of ZFPs and
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Jia Liu Editor
Zinc Finger Proteins Methods and Protocols
Methods
in
M o l e c u l a r B i o lo g y
Series Editor John M. Walker School of Life and Medical Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK
For further volumes http://www.springer.com/series/7651
Zinc Finger Proteins Methods and Protocols
Edited by
Jia Liu Shanghai Institute for Advanced Immunochemical Studies, ShanghaiTech University, Shanghai, China
Editor Jia Liu Shanghai Institute for Advanced Immunochemical Studies ShanghaiTech University Shanghai, China
ISSN 1064-3745 ISSN 1940-6029 (electronic) Methods in Molecular Biology ISBN 978-1-4939-8798-6 ISBN 978-1-4939-8799-3 (eBook) https://doi.org/10.1007/978-1-4939-8799-3 Library of Congress Control Number: 2018953313 © Springer Science+Business Media, LLC, part of Springer Nature 2018 This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed. The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. The publisher, the authors, and the editors are safe to assume that the advice and information in this book are believed to be true and accurate at the date of publication. Neither the publisher nor the authors or the editors give a warranty, express or implied, with respect to the material contained herein or for any errors or omissions that may have been made. The publisher remains neutral with regard to jurisdictional claims in published maps and institutional affiliations. This Humana Press imprint is published by the registered company Springer Science+Business Media, LLC part of Springer Nature. The registered company address is: 233 Spring Street, New York, NY 10013, U.S.A.
Preface Zinc finger proteins (ZFPs) represent the largest family of eukaryotic transcription factors (TFs). ZFPs are engaged in a wide range of biological functions including transcription, DNA methylation, cell cycling, cell division, tumorigenesis, and others. There are distinct classes of ZFPs such as C2H2 fingers, C4 fingers, and CCHC fingers. Despite different sequence organization, all ZFPs are characterized by the tetrahedral coordination of zinc ions (Zn2+) with cysteines and histidines. The DNA-binding preferences of ZFPs have been extensively studied for decades. ZFP can recognize DNA in a relatively simple mode. For example, each C2H2 finger recognizes three or four DNA base pairs via residues −1, +2, +3, and +6 on the α helix of ZFP. Elucidation of th
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