Identification of novel QTLs for late leaf spot resistance and validation of a major rust QTL in peanut ( Arachis hypoga
- PDF / 1,877,361 Bytes
- 13 Pages / 595.276 x 790.866 pts Page_size
- 44 Downloads / 198 Views
ORIGINAL ARTICLE
Identification of novel QTLs for late leaf spot resistance and validation of a major rust QTL in peanut (Arachis hypogaea L.) Suhail Ahmad1,2 · Bhagwat Nawade1 · Chandramohan Sangh1 · Gyan P. Mishra1,3 · Tejas C. Bosamia1 · Radhakrishnan T.1 · Narendra Kumar1 · Jentilal R. Dobaria1 · Harsukh P. Gajera2 Received: 2 March 2020 / Accepted: 21 September 2020 © King Abdulaziz City for Science and Technology 2020
Abstract Co-occurrence of two devastating foliar-fungal diseases of peanut, viz., late leaf spot (LLS), and rust may cause heavy yield loss besides adversely affecting the quality of kernel and fodder. This study reports the mapping of seven novel stressrelated candidate EST-SSRs in a region having major QTLs for LLS and rust diseases using an F2 mapping population (GJG17 × GPBD4) consisting of 328 individuals. The parental polymorphism using 1311 SSRs revealed 84 SSRs (6.4%) as polymorphic and of these 70 SSRs could be mapped on 14 linkage groups (LG). QTL analysis has identified a common QTL (LLSQTL1/RustQTL) for LLS and rust diseases in the map interval of 1.41 cM on A03 chromosome, explaining 47.45% and 70.52% phenotypic variations, respectively. Another major QTL for LLS ( LLSQTL1), explaining a 29.06% phenotypic variation was also found on LG_A03. A major rust QTL has been validated which was found harboring R-gene and resistance-related genes having a role in inducing hypersensitive response (HR). Further, 23 linked SSRs including seven novel EST-SSRs were also validated in 177 diverse Indian groundnut genotypes. Twelve genotypes resistant to both LLS and rust were found carrying the common (rust and LLS) QTL region, LLS QTL region, and surrounding regions. These identified and validated candidate EST-SSR markers would be of great use for the peanut breeding groups working for the improvement of foliar-fungal disease resistance. Keywords Expressed sequence tag · Foliar-fungal disease · Groundnut · LLS · QTL mapping
Introduction Globally, peanut (Arachis hypogaea L.) is one of the most important oilseed crops, having multiple economic uses like vegetable oil, confectionery, and feed (Nawade et al. Electronic supplementary material The online version of this article (https://doi.org/10.1007/s13205-020-02446-4) contains supplementary material, which is available to authorized users. * Gyan P. Mishra [email protected] * Radhakrishnan T. [email protected] 1
Department of Biotechnology, Directorate of Groundnut Research, Junagadh, India
2
Department of Biotechnology, Junagadh Agricultural University, Junagadh, Gujarat, India
3
Division of Genetics, Indian Agricultural Research Institute, New Delhi, India
2019; Bhalani et al. 2019). It is widely cultivated as a grainlegume in Asia, Africa, and America, especially in the arid and semi-arid regions (Nawade et al. 2018). Besides the rich source of oil (40–60%), protein (25–30%), and carbohydrate (10–20%), it also contains various cardio-protective and anti-carcinogenic compounds (Aggarwal et al. 2004; Ko et
Data Loading...
