Mapping novel sources of leaf rust and stripe rust resistance introgressed from Triticum dicoccoides in cultivated tetra
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ORIGINAL ARTICLE
Mapping novel sources of leaf rust and stripe rust resistance introgressed from Triticum dicoccoides in cultivated tetraploid wheat background Ahmed Fawzy Elkot1,2 • Rohtas Singh1 • Satinder Kaur1 • Jaspal Kaur1 • Parveen Chhuneja1 Received: 26 December 2019 / Accepted: 30 August 2020 Ó Society for Plant Biochemistry and Biotechnology 2020
Abstract Wild emmer wheat, Triticum dicoccoides, the progenitor of modern tetraploid and hexaploid wheats, is an important resource for new variability for disease resistance genes. T. dicoccoides accession pau4656 showed resistance against prevailing leaf rust and stripe rust races in India and was used for developing stable introgression lines (IL) in T. durum cv Bijaga yellow and named as IL pau16068. F5 Recombinant inbred lines (F5 RILs) were developed by crossing IL pau16068 with T. durum cultivar PBW114 and RIL population was screened against highly virulent Pt and Pst pathotypes at the seedling and adult plant stages. Inheritance analyses revealed that population segregated for two genes for all stage resistance (ASR) against leaf rust, one ASR gene against stripe rust and three adult plant resistance (APR) genes for stripe rust resistance. For mapping these genes a set of 483 SSR marker was used for bulked segregant analysis. The markers showing diagnostic polymorphism in the resistant and susceptible bulks were amplified on all RILs. Single marker analysis placed all stage leaf rust resistance genes on chromosome 6A and 2A linked to the SSR markers Xwmc256 and Wpaus268, respectively. Likewise one all stage stripe rust resistance gene were mapped on long arm of chromosome 6A linked to markers 6AL-5833645 and 6AL-5824654 and two APR genes mapped on chromosomes 2A and 2B close to the SSR marker Wpaus268 and Xbarc70, respectively. The current study identified valuable leaf rust and stripe rust resistance genes effective against multiple rust races for deployment in the wheat breeding programme. Keywords Leaf rust Stripe rust T. dicoccoides Introgression lines T. durum Bulked segregant analysis Abbreviations Pst Puccinia striiformis Pt Puccinia triticina SSR Simple sequence repeats IL Introgression line QTL Quantitative trait loci RIL Recombinant inbred line BSA Bulked segregant analysis
Electronic supplementary material The online version of this article (https://doi.org/10.1007/s13562-020-00598-1) contains supplementary material, which is available to authorized users. & Parveen Chhuneja [email protected] 1
School of Agricultural Biotechnology, Punjab Agricultural University, Ludhiana 141004, India
2
Wheat Research Department, Agricultural Research Center, Field Crops Research Institute, Giza 12619, Egypt
IT ASR APR
Infection type All stage resistance Adult plant resistance
Introduction Stripe rust, caused by Puccinia striiformis Westend. f. sp. tritici Erikss. (Pst) and leaf rust caused by Puccinia triticina (Pt) are considered the most devastating diseases of wheat in India and worldwide (Todorovska et al. 2009; Lan et al. 2017; Bhardwa
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