Identification of SSR markers which could differentiate blast disease resistance accessions in finger millet ( Eleusine
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J. Crop Sci. Biotech. 2017 (March) 20 (1) : 37 ~ 44 DOI No. 10.1007/s12892-016-0091-2 RESEARCH ARTICLE
Identification of SSR Markers Which Could Differentiate Blast Disease Resistance Accessions in Finger Millet (Eleusine coracana (L.) Gaertn.) Bheema Lingeswara Reddy IN1*, Sivaramakrishnan S2 1
Dept. of Agricultural Biotechnology, Acharya N.G. Ranga Agricultural University (ANGRAU), Rajendranagar, Hyderabad- 500 030 (Telangana), India 2 Rajiv Gandhi Centre for Biotechnology, Poojappura, Thiruvananthapuram - 695 014 Kerala, India Received: September 21, 2016 / Revised: December 16, 2016 / Accepted: March 08, 2017 Ⓒ Korean Society of Crop Science and Springer 2015
Abstract Microsatellites also known as SSR are the class of repetitive DNA sequences present throughout the genome of all eukaryotic organisms. The present study identified SSRs using biotinylated beads capture method. Ten sets of primers were designed based on sequences having at least (CT)10 repeats. A total of 27 accessions having a mix of both African (resistant) and Indian origin (resistant and susceptible) were assessed using 30 microsatellite markers. Amplification products were obtained for all 30 primers studied; 25 out of these primers were found to be polymorphic with 13 primers showing two alleles per locus. The current study identified markers which could differentiate between resistant and susceptible accessions and also segregate accessions based on geographical region. These informative SSR markers can be used in finger millet genetic improvement projects. Key words : Finger millet, microsatellite, marker, diversity
Introduction Finger millet (Eleusine coracana L. Gaertn.) subspecies coracana belongs to the family poaceae. Finger millet (Eleusine coracana (L.) Gaertn.) is an important cereal crop for subsistence agriculture in dry areas of Eastern Africa, India, and Sri Lanka. India is the major producer of finger millet contributing to nearly 60% of the global production. In India, among the various millets cultivated finger millet is ranked next to pearl millet and is cultivated on 2.6 Mha area with a production of about 3.0 mt (www.indiastat.com). The cultivation of finger millet in India is mostly confined to the southern states, Andhra Pradesh, Tamil Nadu, and Karnataka. Finger millet has an excellent nutritional value and is particularly rich in methionine, iron, and calcium (Barbeau and Hilu 1993; Vadivoo et al. 1998). The nutritional value of finger millet makes it an ideal food for children, expectant women, and diabetic patients (National Research Council I.N. Bheema Lingeswara Reddy () Email: [email protected]
The Korean Society of Crop Science
1996). Magnaporthe grisea causes significant reduction in production especially on high-yielding varieties of finger millet. Yield loss is due to inhibition of grain formation/or formed grains being shrivelled which affects yield losses as high as 90% (Ekwamu 1991). Microsatellites are the class of repetitive DNA sequences present in all organisms, both in eukaryotes (Morgante et
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