Evaluation of genetic integrity of pearl millet seeds during aging by genomic-SSR markers
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ORIGINAL ARTICLE
Evaluation of genetic integrity of pearl millet seeds during aging by genomic‑SSR markers Xuming Dan1 · Chengran Wang1 · Yanning Su1 · Ailing Zhang1 · Ruijia Wang1 · Imran Khan1 · Linkai Huang1 Received: 12 February 2020 / Accepted: 3 July 2020 © Springer Nature B.V. 2020
Abstract Seed is an important way to store germplasm resources but its genetic integrity will decrease during long-term preservation. So, it’s essential to update seeds according to the aging level of different species. Pearl millet [Cenchrus americanus (L.) Morrone syn., Pennisetum glaucum (L.) R. Br.] is a crucial forage grass, biofuel plant and important crops in the world bringing huge economic and ecological benefits. However, there is no report about the impact of aging on genetic integrity of its seeds. In this study, four genetic diversity indexes (the percentage of polymorphic bands, PPB; the effective number of alleles, Ne; the Nei’s gene diversity index, H; the Shannon’s information index, I) and 20 pairs of genomic-SSR primers were used to certify the optimal sample volume of pearl millet for molecular study and found that the best sample volume was 60. After the artificial aging test, the germination rate and four genetic diversity parameters (the number of alleles, Na; Ne; H; I) were used to evaluate the change of genetic integrity at different aging levels. The results showed that the germination rate and these four genetic diversity parameters declined with the increase of aging levels. Furthermore, when the germination rate of pearl millet seeds went down to 68.23%, a significant difference in genetic integrity was observed with unaged seeds. In conclusion, the optimal sample size of pearl millet was 60 and the critical point of germination rate to renew germplasm resources was 68.23% and these finds might contribute to the scientific study and the safe conservation of pearl millet. Keywords Pearl millet · Sampling strategy · Seeds aging · Genetic integrity · Genomic-SSR · Conservation
Introduction The germplasm resources are the basis of genetic breeding, biotechnology and plant science. Nowadays, around 61 million germplasm resources have been collected and 90% of which are seeds. Seeds are one of the most common and cost-optimal ways to store germplasm resources for a long time [1]. The condition of seeds is monitored by genetic integrity, which refers to the difference in alleles and the frequency of genotype compared with the original Xuming Dan and Chengran Wang have contributed equally to this work. Electronic supplementary material The online version of this article (https://doi.org/10.1007/s11033-020-05642-w) contains supplementary material, which is available to authorized users. * Linkai Huang [email protected] 1
Department of Grassland Science, Sichuan Agricultural University, Chengdu 611330, Sichuan, China
population. The genetic integrity of cross-pollination plants like pearl millet is mainly affected by seeds aging, population and environment [2]. However, even if the seeds are
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