Immunohistological Detection of Active Satellite Cellsin Rat Dorsal Root Ganglia after Parenteral Administration of Lipo
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Bulletin of Experimental Biology and Medicine, Vol. 169, No. 5, September, 2020 IMMUNOLOGY AND MICROBIOLOGY
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Immunohistological Detection of Active Satellite Cells in Rat Dorsal Root Ganglia after Parenteral Administration of Lipopolysaccharide and during Aging E. A. Kolos and D. E. Korzhevskii
Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 169, No. 5, pp. 596-599, May, 2020 Original article submitted January 28, 2020 Immunohistochemical reaction to glial fibrillar acid protein (GFAP) is widely used for identification of activated satellite cells in sensory ganglia. We used this marker in studies of satellite cells activation in dorsal root ganglia during aging and under conditions of experimental systemic inflammation: in young (4 months) and aged (18-19 months) rats and animals with experimental LPS-induced systemic inflammation. The number of GFAP+ satellite cells increased significantly after parenteral injection of LPS and during aging, which can indicate similarity of mechanisms of reactive glial changes during aging and systemic inflammation. Key Words: satellite cells; dorsal root ganglion; aging; systemic inflammation Glial cells are important accessory elements essential for maintaining the work of nervous structures. Changes in the function of neuroglia in various pathologies and during aging are primarily described for the CNS. Glial cells of the peripheral nervous system, especially satellite cells of the dorsal root ganglion (DRG), are little studied and their contribution to the function of sensory neurons is still not completely understood. DRG contains cell bodies of the primary afferent neurons transmitting sensory information from the periphery to the CNS. The soma and the initial axonal segment of neurons in sensory ganglia of adult vertebrates are surrounded by a layer of satellite cells [8,13] that perform protective, trophic, and regulatory functions and participate in the maintenance of homeostasis of the perineuronal environment, utilization of neurotransmitters, phagocytosis, and apoptosis [13]. Various factors inducing disturbances of the peripheral nervous system homeostasis affect the structure, function, and counts of glial cells in DRG. Experimental injuries (peripheral nerve crossing, compression of DRG, and local inflammation) lead to activation Institute of Experimental Medicine, St. Petersburg, Russia. Address for correspondence: [email protected]. E. A. Kolos
of satellite cells, which manifests by changed ionic composition of the perineuronal space, increase in the number of gap junctions between satellite cells, enhanced production of neurotrophins, cytokines, and TNFα and enhanced GFAP expression by satellite cells [8,13]. In modern studies GFAP is widely used as a reliable marker of satellite cell activation [2,6]. Despite intensive studies of the relationships between nervous and satellite cells in DRG in health and disease, the data on reactive changes in the satellite cells of sensory ganglia under conditions of systemic inflammatory p
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