Morphological Analysis of the Respiratory Tract of Rats after Parenteral Administration of Silicon Dioxide Nanoparticles
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nalysis of the Respiratory Tract of Rats after Parenteral Administration of Silicon Dioxide Nanoparticles G. Yu. Yukina1, I. V. Polovnikov1, E. G. Sukhorukova1, S. G. Zhuravskii1,2, and M. M. Galagudza1,2 Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 170, No. 7, pp. 102-105, July, 2020 Original article submitted April 17, 2020 Morphological analysis of the respiratory tract of Wistar rats was performed after a single pa renteral administration of 12-nm silicon dioxide nanoparticles (1 ml, 2 mg/ml, intravenously) was performed. On day 21 and in 2, 4, and 6 months after the administration of nanoparticles, the development of macrophage infiltration in the interstitium of the respiratory tract was demonstrated by histological and immunohistochemical methods. The pool of alveolar macrophages increased in 4 months after administration (p=0.004) and returned to the control values in 6 months. The number of mast cells did not significantly change at all stages of the experiment. Connective tissue remodeling in the interstitium of the respiratory tract was not observed throughout the observation period. Key Words: respiratory tract of rats; silicon dioxide nanoparticles; macrophages; mast cells; surfactant Silicon dioxide nanoparticles (SDNP) after parenteral administration show high tropism to the tissues of the respiratory tract [2,3]. It is assumed that changes induced by parenterally administered SDNP are similar to pulmonary silicosis that develops after inhalation of SDNP. Moreover, particle size is a factor for the development of toxic injury during macrophage activation and subsequent stimulation of pneumofibrogenesis [10]. The search for optimal particle size determining tropism to the lung tissue and at the same time minimal nanotoxicity is a crucial task in solving the problem of drug delivery. It has been shown that single administration of 150-nm SDNP do not cause gross changes in the lung tissue, but this conclusion was made from the results of experiments with short observation period (14 days) [4]. The aim of the work was to evaluate the morphological changes in the respiratory tract of rats within 6 months after a single parenteral administration of 12-nm SDNP. 1 I. P. Pavlov First St. Petersburg State Medical University, St. Petersburg, Russia; 2V. A. Almazov National Medical Research Centre, Ministry of Health of the Russian Federation, St. Petersburg, Russia. Address for correspondence: [email protected]. E. G. Sukhorukova
MATERIALS AND METHODS The study was performed on 40 somatically healthy male Wistar rats weighing 220-250 g (Rappolovo Animal Nursery) kept under standard conditions. The experiments were performed according to the Good Laboratory Practice standards and European Convention on the Protection of Vertebrate Animals used for Experimental and Other Scientific Purposes (Strasbourg, 1986). Animals were divided into control and experimental groups (20 rats each) that received injection of saline (1 ml) or SDNP suspension (1 ml), respectively. The objects of the study we
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