Impact of herbicide pretilachlor on reproductive physiology of walking catfish, Clarias batrachus (Linnaeus)
- PDF / 242,812 Bytes
- 8 Pages / 547.087 x 737.008 pts Page_size
- 17 Downloads / 227 Views
Impact of herbicide pretilachlor on reproductive physiology of walking catfish, Clarias batrachus (Linnaeus) Rakesh Soni & Sushant Kumar Verma
Received: 20 April 2020 / Accepted: 28 July 2020 # Springer Nature B.V. 2020
Abstract Herbicide pretilachlor is widely used in paddy fields to control annual weeds. The present study has been carried out in walking catfish, Clarias batrachus, to evaluate the impact of herbicide pretilachlor on reproductive physiology after chronic exposure. Based on the median lethal concentration value (96 h), fish were exposed to three nominal test concentrations of pretilachlor ((SL-I (1/20th LC50), SLII (1/15th LC50), and SL-III (1/10th LC50)) for 30, 45, and 60 days after which plasma sex steroid profile, plasma vitellogenin concentration, and gonadal aromatase activity were analyzed in both sexes. Plasma concentration of testosterone decreases in herbicide-exposed male fish. Significant increase in plasma 17β-estradiol, plasma vitellogenin concentration, and gonadal aromatase activity were observed in herbicide-exposed male fish. All these alterations in reproductive parameters in male fish are dependent on concentration and exposure duration of herbicide. On the other hand, significant decrease in plasma concentration of testosterone was observed in female fish which was also dependent on concentration and exposure duration of herbicide. No significant changes in plasma 17β-estradiol concentrations, plasma vitellogenin concentration, and gonadal aromatase activity were observed in female fish. Above findings clearly suggested that herbicide pretilachlor acts as endocrine disruptor in fish and affects overall reproductive R. Soni : S. K. Verma (*) Department of Zoology, Guru Ghasidas Vishwavidyalaya (Central University), Bilaspur, Chattisgarh, India e-mail: [email protected]
physiology of fish, but its ability to induce reproductive toxicity in male and female differs considerably. Keywords Pretilachlor . Clarias batrachus . Testosterone . 17β-estradiol . Vitellogenin . Gonadal aromatase activity
Introduction Herbicides are mostly used in agricultural fields to remove unwanted plants. From agricultural fields, they may enter nearby water systems and impose harmful effects on fish including altered reproductive physiology. Herbicide reduces the reproductive capacity of fish by creating hormonal imbalance (Shioda and Wakabayashi 2000), by disrupting sex steroid metabolism (Moore and Waring 1998) and by altering normal function of hypothalamic pituitary gonadal axis (Li et al. 2009). The chloroacetamide herbicide pretilachlor is widely used in paddy field. It inhibits cell division in herbs by interfering with the normal process of fatty acid synthesis (Kaushik et al. 2006). Chloroacetamide herbicides have been detected in surface water (Hladik et al. 2008), soil (Chao et al. 2007), and sediments (Xue et al. 2005). Some of them are suspected carcinogens (Coleman et al. 2000). They are found to be toxic for fish by several authors from time to time. Butachlor disrupts thyroid
Data Loading...
