Impact of Potential Probiotic Lactobacillus Strains on Host Growth and Development in a Drosophila melanogaster Model
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Impact of Potential Probiotic Lactobacillus Strains on Host Growth and Development in a Drosophila melanogaster Model Pasaraporn Laomongkholchaisri 1,2 & Rawee Teanpaisan 2,3 & Monwadee Wonglapsuwan 4 & Supatcharin Piwat 1,2 Accepted: 27 August 2020 # Springer Science+Business Media, LLC, part of Springer Nature 2020
Abstract Lactobacillus paracasei SD1, Lactobacillus rhamnosus SD11, and Lactobacillus gasseri SD12 were proposed as potential probiotics for oral health. However, the effects of them on host physiology are still unknown. This study aimed to select strains that can promote host growth and development in monocolonized Drosophila model compared with axenic and the commercial Lactobacillus rhamnosus GG–treated flies. The morphogenesis and growth of axenic Drosophila melanogaster were assessed from embryo to adult stage when provided with each probiotic strain–supplemented food. The colonization and persistence of probiotic in fly gut were also evaluated. The results indicated that axenic condition caused the lowest adult weight and emergence rate. All probiotic groups had higher weight than axenic group. Lact. rhamnosus SD11 group presented high adult emergence rate equivalent to Lact. rhamnosus GG group, which is significantly higher than the others. However, Lact. gasseri SD12 group had significantly lower adult weight and emergence rate when compared with Lact. rhamnosus GG group. The gut probiotics levels were rapidly increased within the first day after receiving probiotics. After probiotic cessation, their number in gut decreased and was maintained at low level, except for Lact. gasseri SD12, which completely vanished since day one. In conclusion, Lact. paracasei SD1, Lact. rhamnosus SD11, and Lact. rhamnosus GG can affect morphogenesis and weight of flies when fed since immature stage and have short period gut colonization. The findings of this study could possibly imply comparable healthpromoting effects between Lact. paracasei SD1 and Lact. rhamnosus SD11 to commercial strain in Drosophila model. Keywords Drosophila melanogaster . Lactobacillus . Probiotics . Growth . Development . Emergence rate
Introduction “Probiotics” are live micro-organisms which, when administered in adequate amounts, confer a health benefit to the host [1]. They have gained worldwide use for more than two decades to promote the health of consumers. Many probiotic strains of Lactobacillus have been proposed in the management of the intestinal flora [2, 3] and certain strains of
* Supatcharin Piwat [email protected] 1
Department of Preventive Dentistry, Faculty of Dentistry, Prince of Songkla University, Hat-Yai, Thailand
2
Common Oral Diseases and Epidemiology Research Center, Faculty of Dentistry, Prince of Songkla University, Hat-Yai, Thailand
3
Department of Stomatology, Faculty of Dentistry, Prince of Songkla University, Hat-Yai, Thailand
4
Department of Molecular Biotechnology and Bioinformatics, Faculty of Science, Prince of Songkla University, Hat-Yai, Thailand
Lactobacillus appear to have a significa
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