Improvement of a dry formulation of Pseudomonas protegens SN15-2 against Ralstonia solanacearum by combination of hypero
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Improvement of a dry formulation of Pseudomonas protegens SN15-2 against Ralstonia solanacearum by combination of hyperosmotic cultivation with fluidizedbed drying Xiaobing Wang . Danyan Tang . Wei Wang
Received: 29 October 2019 / Accepted: 4 August 2020 International Organization for Biological Control (IOBC) 2020
Abstract The application value of Pseudomonas protegens in agriculture has been widely studied, but the manufacturing processes used to produce P. protegens limits its usefulness as a biological control agent because of its poor shelf life due to low stress resistance during processing. We combined hyperosmotic cultivation and fluidized-bed drying to prepare much more stable P. protegens preparations. During drying and cryogenic storage, the viability of hyperosmotic cells was significantly improved compared to that of cells prepared by previous methods. Addition of skim milk provided an excellent protective effect for both hyperosmotic and standard cells. In two field trials, when the concentration of the P. protegens preparation was 0.333% w/v, the biocontrol efficacy
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Electronic supplementary material The online version of this article (https://doi.org/10.1007/s10526-020-10042-x) contains supplementary material, which is available to authorized users. X. Wang D. Tang W. Wang (&) State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China e-mail: [email protected] X. Wang e-mail: [email protected] D. Tang e-mail: [email protected]
against tomato bacterial wilt was more than 80%, which was significantly higher than that of the control provided by the antibiotics zhongshengmycin and streptomycin sulfate. The results show that P. protegens products can be successfully prepared by combination of hyperosmotic cultivation and fluidized-bed drying. Keywords Pseudomonas protegens Hyperosmotic cultivation Fluidized-bed drying Biocontrol
Introduction Several fluorescent Pseudomonas strains contribute to the disease suppressiveness of soils (Frapolli et al. 2008) or protecting plants from diseases pathogens when used as biocontrol agents (BCAs) (Raaijmakers et al. 2009). Pseudomonas protegens, formerly part of the Pseudomonas fluorescens complex, is a widespread plant-protecting bacteria producing a specific spectrum of antibiotic compounds, including 2,4diacetylphloroglucinol and pyoluteorin (Ramette et al. 2011). Our laboratory has isolated a strain of P. protegens SN15-2 which produces metabolites that have proven inhibitory effects of tomato bacterial wilt (Lou et al. 2018). Therefore, we set out to develop P. protegens SN15-2 into a biocontrol product that can be widely used in agricultural fields. P. protegens can be considered to be very fragile with weak ability
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to resist high temperature and dehydration and high mortality rate during processing. Although there have been many studies on the efficacy of Pseudomonas as a biologi
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