In infertile women, cells from Chlamydia trachomatis infected site release higher levels of interferon-gamma, interleuki
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BioMed Central
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In infertile women, cells from Chlamydia trachomatis infected site release higher levels of interferon-gamma, interleukin-10 and tumor necrosis factor-alpha upon heat shock protein stimulation than fertile women Pragya Srivastava1, Rajneesh Jha1, Sylvette Bas2, Sudha Salhan3 and Aruna Mittal*1 Address: 1Institute of Pathology – ICMR, Safdarjung Hospital Campus, Post Box no. 4909, New Delhi-110 029, India, 2Division of Rheumatology, Department of Internal Medicine, University Hospital, 1211 Geneva 14, Switzerland and 3Department of Gynecology & Obstetrics, Safdarjung Hospital, New Delhi-110 029, India Email: Pragya Srivastava - [email protected]; Rajneesh Jha - [email protected]; Sylvette Bas - [email protected]; Sudha Salhan - [email protected]; Aruna Mittal* - [email protected] * Corresponding author
Published: 20 May 2008 Reproductive Biology and Endocrinology 2008, 6:20
doi:10.1186/1477-7827-6-20
Received: 17 March 2008 Accepted: 20 May 2008
This article is available from: http://www.rbej.com/content/6/1/20 © 2008 Srivastava et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract Background: The magnitude of reproductive morbidity associated with sexually transmitted Chlamydia trachomatis infection is enormous. Association of antibodies to chlamydial heat shock proteins (cHSP) 60 and 10 with various disease sequelae such as infertility or ectopic pregnancy has been reported. Cellmediated immunity is essential in resolution and in protection to Chlamydia as well as is involved in the immunopathogenesis of chlamydial diseases. To date only peripheral cell mediated immune responses have been evaluated for cHSP60. These studies suggest cHSPs as important factors involved in immunopathological condition associated with infection. Hence study of specific cytokine responses of mononuclear cells from the infectious site to cHSP60 and cHSP10 may elucidate their actual role in the cause of immunopathogenesis and the disease outcome. Methods: Female patients (n = 368) attending the gynecology out patient department of Safdarjung hospital, New Delhi were enrolled for the study and were clinically characterized into two groups; chlamydia positive fertile women (n = 63) and chlamydia positive infertile women (n = 70). Uninfected healthy women with no infertility problem were enrolled as controls (n = 39). cHSP60 and cHSP10 specific cytokine responses (Interferon (IFN)-gamma, Interleukin (IL)-10, Tumor Necrosis Factor (TNF)-alpha, IL13 and IL-4) were assessed by ELISA in stimulated cervical mononuclear cell supernatants. Results: cHSP60 and cHSP10 stimulation results in significant increase in IFN-gamma (P = 0.006 and P = 0.04 respectively) and IL-10 levels (P = 0.04) in infertile group as c
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