In Vitro Characterization of Lactic Acid Bacteria Isolated from Bovine Milk as Potential Probiotic Strains to Prevent Bo
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In Vitro Characterization of Lactic Acid Bacteria Isolated from Bovine Milk as Potential Probiotic Strains to Prevent Bovine Mastitis Matías S. Pellegrino 1,2 & Ignacio D. Frola 1 & Berardo Natanael 1,2 & Dino Gobelli 1 & María E.F. Nader-Macias 2,3 & Cristina I. Bogni 1
# Springer Science+Business Media, LLC, part of Springer Nature 2018
Abstract Bovine mastitis causes economic losses on dairy farms worldwide. Lactic acid bacteria (LAB) in animal health are an alternative tool to avoid antibiotic therapy on the prevention of bovine mastitis. In previous studies, 12 LAB isolated from bovine milk were selected taking into account some of the following characteristics: hydrophobicity, auto aggregative capability, inhibition of indicator pathogens, hydrogen peroxide, and capsular polysaccharide production. These LAB were considered because of their beneficial properties. In this work, we also analyzed the antimicrobial activity and the co-aggregation against mastitis causing bacteria, auto-inhibition, adhesion to bovine teat canal epithelial cells (BTCEC), and growth kinetic curves for the 12 LAB. Two of them, Lactococcus lactis subsp. lactis CRL 1655 and Lactobacillus perolens CRL 1724, were selected because they had an interesting pattern of adhesion to BTEC, the inhibition of pathogens and the co-aggregation with the 100% of the assayed pathogens. They showed a predictable difference in the PFGE genomic pattern bands. The kinetic growth of these two strains was similar between them and with the rest of the assayed LAB. The strains selected in the present study showed indispensable characteristics for their inclusion in a probiotic formulation to be used at dry-off period for the prevention of bovine mastitis. Keywords Lactic acid bacteria . Probiotic . Bovine mastitis . Prevention
Introduction Bovine mastitis is defined as the inflammation of the mammary gland where the tissue of the udder is severely affected. It is considered as the major endemic and prevalent disease of dairy cattle [1–3] and is generally caused by microorganisms [4–6]. In approximately 70% of mastitis cases, microorganisms can be isolated. In the most cases, the infection occurs when bacteria get into the udder through the teat canal and multiply causing an inflammation with or without clinical * Matías S. Pellegrino [email protected] 1
Departamento de Microbiología e Inmunología, Facultad de Ciencias Exactas, Físico-Químicas y Naturales, Universidad Nacional de Río Cuarto, Ruta 36 km 601, 5800-Río Cuarto, Cordoba, Argentina
2
Member of Consejo Nacional de Investigaciones Científicas y Tecnológicas (CIC-CONICET), Buenos Aires, Argentina
3
Departamento de Microbiología Preventiva, CERELA-CONICET (Centro de Referencia para Lactobacilos-Consejo Nacional de Investigaciones Científicas y Técnicas de Argentina), Chacabuco 145, 4000 San Miguel de Tucuman, Argentina
signs (clinical or subclinical mastitis, respectively) [1]. The disease causes considerable distress on the animal, a decreased milk production, and major economic losses
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