Investigation of Lactic Acid Bacteria Isolated from Giant Panda Feces for Potential Probiotics In Vitro
- PDF / 586,760 Bytes
- 7 Pages / 595.276 x 790.866 pts Page_size
- 51 Downloads / 219 Views
Investigation of Lactic Acid Bacteria Isolated from Giant Panda Feces for Potential Probiotics In Vitro Qian Liu 1 & Xueqin Ni 1 & Qiang Wang 2 & Zhirong Peng 1 & Lili Niu 2 & Meiling Xie 1 & Yicen Lin 1 & Yi Zhou 1 & Hao Sun 3 & Kangcheng Pan 3 & Bo Jing 3 & Dong Zeng 1,3
# Springer Science+Business Media, LLC, part of Springer Nature 2018
Abstract The present study aimed to isolate an optimal lactic acid bacterial strain from the feces of healthy giant pandas. The strain exhibited good stability at low pH and high bile salt concentrations, activity against pathogens relevant to pandas, and antibiotic susceptibility. In the current study, 25 isolates were obtained from de Man, Rogosa, and Sharpe agar. Two (E21 and G83) and eight (E1, E2, E16, E18, E21, E69, E70, and G83) isolates demonstrated good performance at pH 2.0 and bile 2% (w/v), respectively. Three isolates (G83, G88, and G90) possessed better antimicrobial effect on enterotoxigenic Escherichia coli CVCC196 (ETEC) than the rest. One isolate (G83) strongly affected Salmonella, whereas three (G83, G87, and G88) exhibited inhibitory activity against Staphylococcus aureus. All isolates were multi-drug resistant. These isolates were identified as Lactobacillus (5 isolates) and Enterococcus (20 isolates) by 16S rRNA sequencing. Virulence genes were detected in Enterococcus isolates. Isolate G83 was identified as Lactobacillus plantarum and was considered as the best probiotic candidate among all of the experimental isolates. This study provided necessary and important theoretical guidance for further experiments on G83 in vivo. Keywords Giant panda . Probiotic . Lactic acid bacteria . Isolation
Introduction The giant panda, Ailuropoda melanoleuca, is famous as a living fossil. It is a vulnerable, endemic species that is extremely popular worldwide. Conservation strategies have resulted in the survival of about 1800 giant pandas worldwide. The giant panda is a herbivore that has retained a typical carnivorous digestive system. It is easily afflicted by various intestinal diseases when the feed structure changes. Under
Xueqin Ni, Qiang Wang and Zhirong Peng are joint first authors. * Dong Zeng [email protected] 1
Animal Microecology Institute, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China
2
Chengdu Wildlife Institute, Chengdu Zoo, Chengdu 610081, China
3
Key Laboratory of Animal Disease and Human Health of Sichuan Province, Chengdu 611130, China
conditions of captivity, the prevention and treatment of diseases rely on antibiotics. However, scholars sought alternative treatments because of the disadvantages of antibiotics. One of the best choices is probiotics, which maintain or restore normal gut microbiota, inhibit pathogen invasion, and prevent inflammation [1–3]. Studies on the effects of intestinal probiotics on giant pandas focused mostly on cellulolytic bacterium and bacillus [4–6]. The present work is the first to investigate lactic acid bacteria (LAB) isolated from the giant panda feces, thereby providing
Data Loading...
