In vitro detection of water contaminants using microfluidic chip and luminescence sensing platform
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RESEARCH PAPER
In vitro detection of water contaminants using microfluidic chip and luminescence sensing platform Bhavishya B. Waghwani1 · Suroosh S. Ali1 · Shubham C. Anjankar1 · Suresh S. Balpande1 · Puspen Mondal2 · Jayu P. Kalambe1 Received: 2 April 2020 / Accepted: 12 August 2020 © Springer-Verlag GmbH Germany, part of Springer Nature 2020
Abstract This work describes the modern development of an autonomous platform for the measurement of phosphate and nitrogen levels in the water. Microfluidic-based framework developed for the identification of contaminants in water as, it has enormous potential in making chip portable, low cost, and miniaturized commercial device. The total framework uses an embedded system and a microfluidic chip. This microfluidic chip is made of polydimethylsiloxane (PDMS) and has been fabricated by X-ray (XRL) lithography techniques. The created framework contains liquid handling with luminescence information investigation, which is important to play out the phosphate and nitrogen estimation. Tests are performed for the detection of phosphate, nitrite, and nitrate utilizing explicit synthetic reagents and tests for each. The created colorimetric-implanted framework can utilized for any liquid investigation like zinc, copper, and lead contaminant detection that usually found in contaminated water. This sort of framework is alluring for use in creating nations, in the field, and rustic regions.
1 Introduction Higher levels of nutrient loading in both fresh and marine ecosystems can have negative impacts on aquatic ecosystems (Zamparas et al. 2014). Though, phosphate is an important compound in the biotic world, because it is necessary for deoxyribonucleic acid (DNA) and adenosine triphosphate (ATP) synthesis. Furthermore, it is one of the important nutrients for plants, but at high concentrations, it creates a condition called eutrophication with the rapid growth of the plant population (algal blooms) in aquatic environments (Cordell et al. 2009, Dupas et al. 2015). Eutrophication as a result of excessive levels of nutrients can result in the formation of harmful algal blooms reduced * Suresh S. Balpande [email protected] Bhavishya B. Waghwani [email protected] Jayu P. Kalambe [email protected] 1
Shri Ramdeobaba College of Engineering and Management, Nagpur, India
Lithography and Microscopy Lab, Synchrotrons Utilization Section, Raja Ramanna Centre for Advanced Technology, Indore, India
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levels of oxygen, the release of algal toxins, the formation of dead zones, and contamination of drinking water supplies (Johnson et al. 2008). The process developed by (Blaen et al. 2016) is not only labor-intensive and costly, but also provides limited regarding the spatial and temporal variation of the nutrient within the water body. Real-time monitoring provides reliable in situ measurements, which can provide spatial data that can enhance our understanding of the processes that drive increases in nutrient levels. The data analysis by these processes can be utilized for enhancing th
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