In Vitro Embryogenesis in Higher Plants
This volume presents an overview of recent advances, innovative applications, and future prospects of in vitro embryogenesis in higher plants. The book's chapters are divided into five parts: Part I contains reviews on general topics (microspore; zygotic
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Maria Antonietta Germanà Maurizio Lambardi Editors
In Vitro Embryogenesis in Higher Plants
METHODS
IN
MOLECULAR BIOLOGY
Series Editor John M. Walker School of Life and Medical Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK
For further volumes: http://www.springer.com/series/7651
In Vitro Embryogenesis in Higher Plants Edited by
Maria Antonietta Germanà Dipartimento Scienze Agrarie e Forestali, Università degli Studi di Palermo, Palermo, Italy
Maurizio Lambardi IVALSA/Trees and Timber Institute, National Research Council (CNR), Sesto Fiorentino, Florence, Italy
Editors Maria Antonietta Germanà Dipartimento Scienze Agrarie e Forestali Università degli Studi di Palermo Palermo, Italy
Maurizio Lambardi IVALSA/Trees and Timber Institute National Research Council (CNR) Sesto Fiorentino, Florence, Italy
ISSN 1064-3745 ISSN 1940-6029 (electronic) Methods in Molecular Biology ISBN 978-1-4939-3060-9 ISBN 978-1-4939-3061-6 (eBook) DOI 10.1007/978-1-4939-3061-6 Library of Congress Control Number: 2015952782 Springer New York Heidelberg Dordrecht London © Springer Science+Business Media New York 2016 This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed. The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. The publisher, the authors and the editors are safe to assume that the advice and information in this book are believed to be true and accurate at the date of publication. Neither the publisher nor the authors or the editors give a warranty, express or implied, with respect to the material contained herein or for any errors or omissions that may have been made. Cover image: Somatic embryogenesis in sweet orange (Citrus sinensis (L.) Osbeck). Photo of Maria Antonietta Germanà Printed on acid-free paper Humana Press is a brand of Springer Springer Science+Business Media LLC New York is part of Springer Science+Business Media (www.springer.com)
Dedication To Emanuela, Maria Luisa, Antonio and Gabriele, the best experiments of my life Maria Antonietta Germanà To my beloved children Matilde and Tommaso Maurizio Lambardi
Foreword I was fortunate to start my research career in plant tissue culture in the 1970s when this field of research was expanding rapidly. The next few decades witnessed an exponential growth in knowledge, understanding, and application of many tissue culture protocols to a wide range of plant species. Then followed a period in the 1990s and turn
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