In vitro evaluation of copper release from MRI-visible, PLGA-based nanospheres
- PDF / 1,542,581 Bytes
- 13 Pages / 595.276 x 790.866 pts Page_size
- 20 Downloads / 170 Views
In vitro evaluation of copper release from MRI-visible, PLGA-based nanospheres Iris Sonia Weitz1,*
, Or Perlman2,3
, Haim Azhari2
, and Sarit Sara Sivan1
1
Department of Biotechnology Engineering, ORT Braude College, 2161002 Karmiel, Israel Department of Biomedical Engineering, Technion–Israel Institute of Technology, Technion City, 3200003 Haifa, Israel 3 Present address: Athinoula A. Martinos Center for Biomedical Imaging, Department of Radiology, Massachusetts General Hospital and Harvard Medical School, Charlestown, MA, USA 2
Received: 23 June 2020
ABSTRACT
Accepted: 22 August 2020
Copper nanostructures offer remarkable therapeutic potential. In particular, copper oxide nanoparticles (CuO NPs) are increasingly studied for diagnostic and therapeutic purposes. Their anticancer potential, as well as contrast-enhancing capabilities for magnetic resonance imaging (MRI) and ultrasound (US), were previously demonstrated. To further exploit their unique multifunctional capabilities, here CuO NPs were successfully embedded in nanocarriers composed of poly(lactic-co-glycolic acid) (PLGA) and poly(ethylene–glycol)-blockpoly(lactide-co-glycolide) (PEG-PLGA) and their release kinetics were studied. The CuO NPs-loaded nanospheres (NS) were obtained with a loading capacity of 3.6% ± 0.6 and 5.6% ± 0.8 respectively, and the copper in vitro release was analyzed over a period of 36 days. Copper release from PLGA-NS was found to follow a zero-order model, while that from PEG-PLGA-NS was best described by the tri-phasic release profile. The Higuchi model showed a higher correlation value (R2) for PLGA-NS and PEG-PLGA-NS (R2 = 0.994 and R2 = 0.959, respectively), suggesting that the release mechanism of CuO NPs is diffusioncontrolled. In addition, CuO NPs, encapsulated within these polymeric nanospheres, retained their visibility, as demonstrated by T1-weighted MRI. Our results provide further insights into the benefit of using CuO NPs-loaded PLGA-based nanospheres as multifunctional nanomaterials, which may be considered as a future theranostic agent for multiple applications including drug delivery and imaging.
Published online: 16 September 2020
Ó
Springer Science+Business
Media, LLC, part of Springer Nature 2020
Handling Editor: Shen Dillon.
Address correspondence to E-mail: [email protected]
https://doi.org/10.1007/s10853-020-05296-w
719
J Mater Sci (2021) 56:718–730
GRAPHIC ABSTRACT
Introduction Reducing complexity in formulation design potentially shortens the gap between the laboratory and clinical trials [1]. The presence of a copper component endows a unique combination of physicochemical properties, and, together with its familiar biological activities, forms nanostructures with inherent multifunctionality [2, 3], becoming a preferable choice for therapeutic and diagnostic delivery in a single ‘‘onefor-all’’ formulation [4]. Copper-based nanoparticles have received considerable attention, mainly due to their potential suitability for various biomedical applications [5]. The most frequently studied
Data Loading...
