In vitro evaluation of Enterococcus faecalis biofilm with laser fluorescence after exposure to endodontic irrigants
- PDF / 399,262 Bytes
- 8 Pages / 595.276 x 790.866 pts Page_size
- 61 Downloads / 179 Views
ORIGINAL ARTICLE
In vitro evaluation of Enterococcus faecalis biofilm with laser fluorescence after exposure to endodontic irrigants Elise Fagundes Cappelletti 1 & Pauline Mastella Lang 2 & Clarissa Cavalcanti Fatturi Parolo 3 & Adrian Lussi 4 & Luciano Casagrande 5 & Francisco Montagner 6 & Jonas Almeida Rodrigues 5 Received: 20 August 2019 / Accepted: 8 October 2020 # Springer Nature Switzerland AG 2020
Abstract Purpose The aim of this study was to evaluate the performance of the DIAGNOdent Pen (KaVo, Biberach, Germany) with respect to the fluorescence detection and quantification of microorganisms after the use of root canal solutions. Methods Forty roots from extracted bovine incisors were divided into four groups (n = 10) according to the contamination time (7 or 14 days) and irrigating solution (5.25% sodium hypochlorite (NaOCl) or 2% chlorhexidine (CHX)). Prior to contamination, the fluorescence of root dentin were evaluated at three depths (3, 8, and 12 mm). The roots were autoclaved and contaminated with Enterococcus faecalis (E. faecalis). After contamination and the use of irrigating solutions, the number of colony-forming units (CFUs) and the fluorescence were determined. Results The number of CFUs obtained in 14-day biofilms was higher (P < 0.05). The fluorescence values were higher for 14-day biofilms than 7-day biofilms in the medium and apical thirds and at baseline (no contamination) (P < 0.05). The 2% CHX produced a greater reduction in the fluorescence values in 7-day biofilms (P < 0.05). Conclusion The DIAGNOdent pen was able to detect and quantify different levels of fluorescence after the use of root canal solutions. Chemical solutions were effective in reducing the microbial load in biofilms of E. faecalis. Keywords Diagnosis . Endodontics . Enterococcus faecalis . Irrigation . Laser fluorescence
Introduction Biofilms are sessile microbial communities that are concentrated in an interface and enclosed in an abundant extracellular * Jonas Almeida Rodrigues [email protected] 1
Federal University of Rio Grande do Sul – Porto Alegre, Porto Alegre, RS, Brazil
2
Integrated Regional University of Alto Uruguai and Missões – Erechim, Porto Alegre, RS, Brazil
3
Department of Social and Preventive Dentistry, Federal University of Rio Grande do Sul – Porto Alegre, Porto Alegre, RS, Brazil
4
Department of Operative, Preventive and Paediatric Dentistry, University of Bern, Bern, Switzerland
5
Paediatric Division, Department of Surgery and Orthopedics, Federal University of Rio Grande do Sul – Porto Alegre, Rua Ramiro Barcellos, 2492. – Bairro Santana, Porto Alegre, RS, Brazil
6
Endodontic Division, Department of Conservative Dentistry, Federal University of Rio Grande do Sul – Porto Alegre, Porto Alegre, RS, Brazil
polymeric matrix [1]. The presence of an extracellular matrix [1], the interspecies interactions in multispecies bacterial biofilms [2], and the ability to adapt to a changing environment [2, 3] stand out as properties of biofilms that are responsible for their resistance to ma
Data Loading...
