In vitro micropropagation of Basella rubra L. through proliferation of axillary shoots
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RESEARCH NOTE
In vitro micropropagation of Basella rubra L. through proliferation of axillary shoots Sandopu Sravan Kumar1,2 · Parvatam Giridhar1,2 Received: 18 December 2019 / Accepted: 24 October 2020 © Springer Nature B.V. 2020
Abstract In vitro micropropagation protocol for Basella rubra regeneration was tried through proliferation of axillary shoots of the potted mature plant. The improved seed germination (70%) was recorded upon 2% urea treatment. The nodal shoot segments from matured potted plant were used to initiate the multiple shoot proliferation. The shoot segments exhibited 70% shoot initiation when cultured on Murashige and Skoog (MS) medium supplemented with Indole-3-acetic acid (IAA) + N6 – Benzylaminopurine (BAP) (0.25 + 2.0 mg/L) and BAP + Kinetin (Kin) (2.0 + 0.5 mg/L) respectively. Multiple shoots (5–6) were obtained on MS medium supplemented with BAP + Kin and IAA + BAP respectively. When compared with silver nitrate (AgNO3) (2–40 µM) and activated charcoal (AC) (0.1–1.0%), the MS medium devoid of any plant growth regulator showed good number of shoots (5.48 ± 2.42), elongation (15.64 ± 2.42 cm) and root length (14.52 ± 2.78 cm). Upon transferring of regenerated microshoots to MS medium, simultaneous elongation of shoots with more shoot number, shoot length and rooting was achieved during four subcultures that carried out at 6 weeks’ interval. The regenerated in vitro shoots showed 100% rooting in MS medium and also in MS medium supplemented with 0.1–1.0% AC. Hundred percent survival of micropropagated shoots well rooted was established successfully under greenhouse condition and the plants were subsequently acclimatized and transferred to the field conditions wherein 90% success rate was noted. Key message We established a novel, and efficient protocol for in vitro B. rubra shoot cultures further multiplication in MS basal medium without any plant growth regulators with 100% elongation and rooting of microshoots that will overcome the use of synthetic hormones for improved micropropagation. Keywords Micropropagation · Adventitious rooting · Microshoots · Germination
Introduction
Communicated by Sergey V Dolgov. Electronic supplementary material The online version of this article (https://doi.org/10.1007/s11240-020-01960-w) contains supplementary material, which is available to authorized users. * Parvatam Giridhar [email protected] 1
Academy of Scientific and Innovative Research (AcSIR), Ghaziabad 201002, India
Plant Cell Biotechnology Department, Council of Scientific and Industrial Research – Central Food Technological Research Institute, Mysore 570020, India
2
Basella rubra L. (Basellaceae) is well-known as Malabar spinach/Ceylon spinach is a green leafy vegetable exploited for its medicinal properties in traditional medicine (Toshiyuki et al. 2001). The two common species of Basella spp. include a red twiner with red leaves B. rubra and green twiner with green leaves B. alba (Khare 2004). The succulent ovate thick leaves and tender stems of the Basella spp. are consu
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