First report of Basella rugose mosaic virus infecting Basella alba in Nigeria
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First report of Basella rugose mosaic virus infecting Basella alba in Nigeria Adedapo Olutola Adediji 1
&
Gabriel Ifo Atiri 1
Received: 28 January 2020 / Accepted: 4 February 2020 # Società Italiana di Patologia Vegetale (S.I.Pa.V.) 2020
Keywords Malabar spinach . Potyvirus . Coat protein
Malabar spinach (Basella alba) is a popular potherb in Nigeria, grown in commercial farms and around household gardens. In 2018, virus-like symptoms such as leaf malformation and mild mosaic were observed on B. alba plants in a backyard garden in Ibadan, Oyo State, Nigeria. Six symptomatic leaf samples were obtained from three plants and three of them tested positive in DAS-ELISA to monoclonal antibodies (Agdia Inc., Elkhart, USA) specific to potyviruses. Total nucleic were extracted from infected plants using the cetyl trimethyl ammonium bromide method and RT-PCR was performed using degenerate primers PotyCP (5’TGGTGYATGANAAYGGNACNT-3’) and Oligo-dT-potyR (5’-CCCAGTCACGACTTTTTTTTTT-3’) (Sudheera et al. 2014) to amplify a partial coat protein (CP) gene and the 3’ end untranslated region of the potyvirus genomic RNA. Products of approximately 800 bp were amplified and an amplicon from a sample, Dg-Ke, was bi-directionally sequenced. The poly(A) tail was trimmed from the sequence and deposited in GenBank under accession number MN996303. Nucleotide and amino acid sequence analysis of the partial CP gene showed 96.1% and 97.9% identities, respectively, with an isolate of Basella rugose mosaic virus (BaRMV-BR; DQ821939) infecting B. rubra in Taiwan
* Adedapo Olutola Adediji [email protected] Gabriel Ifo Atiri [email protected] 1
Department of Crop Protection and Environmental Biology, Faculty of Agriculture, University of Ibadan, Ibadan, Oyo State, Nigeria
(Huang and Chang 2006). To our knowledge, this is the first report of a potyvirus infecting B. alba in Nigeria. Although a cucumber mosaic virus isolate has recently been reported from B. alba in Nigeria (Adediji 2019), the presence of BaRMV suggests that the plant is a host to multiple viruses.
References Adediji AO (2019) Molecular detection of Cucumber mosaic virus from Basella alba, Telfairia occidentalis and Talinum fruticosum in Nigeria. J Plant Prot Res 59(2):177–184. https://doi.org/10.24425/ jppr.2019.129282 Huang CH, Chang YC (2006) Basella rugose mosaic virus, a new potyvirus infects Basella rubra. Plant Pathol 55:819. https://doi. org/10.1111/j.1365-3059.2006.01477.x Sudheera Y, Vishnu Vardhan GP, Hema M, Krishna Reddy M, Sreenivasulu P (2014) Characterization of a potyvirus associated with yellow mosaic disease of jasmine (Jasminum sambac L.) in Andhra Pradesh, India. Vir Dis 25(3):394–397. https://doi.org/10. 1007/s13337-014-0193-0 Publisher’s note Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
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