Increased expression of miR-601 is associated with poor prognosis and tumor progression of gastric cancer
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RESEARCH
Open Access
Increased expression of miR-601 is associated with poor prognosis and tumor progression of gastric cancer Cuili Min*†, Aixia Zhang† and Jing Qin
Abstract Background: MicroRNAs (miRNAs) have been considered to participate in many tumorigenesis, including gastric cancer (GC). Abnormal expression of miR-601 has been reported in GC, but its role is not clear. The goal of this study is to explore the expression patterns, clinical value and functional role of miR-601 in GC. Methods: Quantitative real-time polymerase chain reaction (qRT-PCR) was conducted to evaluate the expression level of miR-601. The association between miR-601 expression and overall survival was estimated by the KaplanMeier survival method. The significance of different variables with respect to survival was analyzed by using the Cox regression assay. Cell experiments were applied to investigate the functional role of miR-601 in GC. Results: We found that miR-601 was significantly up-regulated in GC tissues and cells compared with the controls (all P < 0.01). The levels of miR-601 expression were significantly associated with TNM stage, lymph node metastasis, lymphatic invasion, and distant metastasis (all P < 0.05). Kaplan-Meier survival analysis showed that patients in the high miR-601 expression group had poor overall survival (log-rank P = 0.001). Moreover, we confirmed that miR-601, TNM stage, and distant metastasis were independent prognostic factors for GC patients. Overexpression of miR-601 in AGS and SGC-7901 cells by miR-601 mimic transfection significantly promoted the cell proliferation, migration, and invasion (P < 0.05). Conclusions: The expression level of miR-601 is dramatically up-regulated in GC. The overexpression of miR-601 promotes the tumor progression of GC, and may be a novel prognostic factor for poor survival in GC patients. Keywords: MicroRNA-601, Prognosis, Progression, Gastric cancer
Introduction Gastric cancer (GC) is one of the most common malignant tumors, and it has become the leading cause of cancer-related death [1, 2]. Although the incidence of GC has a dropping tendency worldwide, it remains higher in China [3, 4]. At present, surgery is the most common method of GC treatment, as well as a radical type of treatment [5]. In recent years, with the wide use of gastric endoscopy, the early diagnosis and treatment of GC have been improved [6]. However, as a result of the tumor recurrence, invasion, and metastasis, the prognosis of GC is still very poor. Thus, it is urgent to * Correspondence: [email protected] † Cuili Min and Aixia Zhang contributed equally to this work. Department of Laboratory Medicine, Yidu Central Hospital of Weifang, No.4138, South Linglongshan Road, Shandong 262500, China
find novel potential molecule biomarkers for early diagnosis and accurate prediction of prognosis. MicroRNAs (miRNAs), are a class of endogenous, conserved and small non-coding RNA molecules, and regulate gene expression at the post-transcriptional level via binding to the 3′- untranslated region (UTR)
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