Increasing l -lysine production in Corynebacterium glutamicum by engineering amino acid transporters
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ORIGINAL ARTICLE
Increasing l‑lysine production in Corynebacterium glutamicum by engineering amino acid transporters Jing Xiao1 · Datao Wang1 · Lei Wang2 · Yanjun Jiang1 · Le Xue1 · Songsen Sui4 · Jianbin Wang4 · Chuanzhuang Guo4 · Ruiming Wang1 · Junqing Wang1 · Nan Li3 · Han Fan1 · Maocui Lv1 Received: 19 March 2020 / Accepted: 17 September 2020 © Springer-Verlag GmbH Austria, part of Springer Nature 2020
Abstract Corynebacterium glutamicum has a long and successful history in the biotechnological production of l-lysine. Besides the adjustment of metabolic pathways, intracellular and extracellular transport systems are critical for the cellular metabolism of l-lysine or its by-products. Here, three amino acid transmembrane transporters, namely, GluE, BrnE/BrnF, and LysP, which are widely present in C. glutamicum strains, were each investigated by gene knockout. In comparison with that in the wild-type strain, the yield of l-lysine increased by 9.0%, 12.3%, and 10.0% after the deletion of the gluE, brnE/brnF, and lysP genes, respectively, in C. glutamicum 23,604. Moreover, the amount of by-product amino acids decreased significantly when the gluE and brnE/brnF genes were deleted. It was also demonstrated that there was no effect on the growth of the strain when the gluE or lysP gene was deleted, whereas the biomass of C. glutamicum WL1702 (ΔbrnE/ΔbrnF) in the fermentation medium was significantly reduced in comparison with that of the wild type. These results also provide useful information for enhancing the production of l-lysine or other amino acids by C. glutamicum. Keywords gluE · brnE/brnF · lysP · Gene knockout · Amino acid transmembrane transporter · l-Lysine
Communicated by S. Stuchlík. Jing Xiao and Datao Wang, the two first authors, contributed equally to this work. Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00726-020-02893-6) contains supplementary material, which is available to authorized users. * Junqing Wang [email protected] * Nan Li [email protected] 1
State Key Laboratory of Biobased Material and Green Papermaking (LBMP), Qilu University of Technology), Jinan 250353, Shandong, People’s Republic of China
2
School of Biotechnology, Jiangnan University, 1800 Lihu Road, Wuxi 214122, Jiangsu, People’s Republic of China
3
College of Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, People’s Republic of China
4
Zhucheng Dongxiao Biotechnology Co., Ltd, Xinxing Town, Zhucheng 262200, Shandong, People’s Republic of China
Abbreviations AcCoA Acetyl coenzyme A Ala l-Alanine Asn l-Asparagine Asp l-Aspartate AspSa Aspartate semialdehyde Chor Chorismate Cit Citrate Fum Fumaric acid G6P Glucose 6-phosphate GDL Gluconolactone Glc Glucose Gln l-Glutamine Glu l-Glutamate Gluc d-Gluconic acid Gluc6P 6-Phospho-d-gluconate Glx Glyoxylate Gly l-Glycine Hcys Homocysteine His l-Histidine Hser Homoserine ICit Isocitrate Ile l-Isoleucine αKG α-Ketoglutarate
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