Induced sputum metabolomic profiles and oxidative stress are associated with chronic obstructive pulmonary disease (COPD
- PDF / 1,459,480 Bytes
- 15 Pages / 595.276 x 790.866 pts Page_size
- 83 Downloads / 252 Views
RESEARCH
Induced sputum metabolomic profiles and oxidative stress are associated with chronic obstructive pulmonary disease (COPD) severity: potential use for predictive, preventive, and personalized medicine Tao Zhu 1 & Shanqun Li 2 & Jiajia Wang 3 Hong Ji 4 & Zhihong Chen 2
&
Chunfang Liu 2 & Lei Gao 2 & Yuzhen Zeng 2 & Ruolin Mao 2
&
Bo Cui 2 &
Received: 11 July 2020 / Accepted: 19 October 2020 # The Author(s) 2020
Abstract Chronic obstructive pulmonary disease (COPD) is a highly heterogeneous disease, and metabolomics plays a hub role in predictive, preventive, and personalized medicine (PPPM) related to COPD. This study thus aimed to reveal the role of induced sputum metabolomics in predicting COPD severity. In this pilot study, a total of 20 COPD patients were included. The induced sputum metabolites were assayed using a liquid chromatography-mass spectrometry (LC-MS/MS) system. Five oxidative stress products (myeloperoxidase (MPO), superoxide dismutase (SOD), glutathione (GSH), neutrophil elastase (NE), and 8-isoPGF2α) in induced sputum were measured by ELISA, and the metabolomic profiles were distinguished by principal component analysis (PCA) and orthogonal projections to latent structures discriminant analysis (OPLS-DA). The Kyoto Encyclopedia of Genes and Genomes (KEGG) was used for pathway enrichment analysis, and a significant difference in induced sputum metabolomics was observed between moderate and severe COPD. The KEGG analysis revealed that the glycerophospholipid metabolism pathway was downregulated in severe COPD. Due to the critical role of glycerophospholipid metabolism in oxidative stress, significant negative correlations were discovered between glycerophospholipid metabolites and three oxidative stress products (SOD, MPO, and 8-iso-PGF2α). The diagnostic values of SOD, MPO, and 8-iso-PGF2α in induced sputum were found to exhibit high sensitivities and specificities in the prediction of COPD severity. Collectively, this study provides the first identification of the association between induced sputum metabolomic profiles and COPD severity, indicating the potential value of metabolomics in PPPM for COPD management. The study also reveals the correlation between glycerophospholipid metabolites and oxidative stress products and their value for predicting COPD severity. Tao Zhu and Shanqun Li contributed equally to this work. Supplementary Information The online version contains supplementary material available at https://doi.org/10.1007/s13167-02000227-w. * Hong Ji [email protected] * Zhihong Chen [email protected] Tao Zhu [email protected] Shanqun Li [email protected] Jiajia Wang [email protected] Chunfang Liu [email protected]
Lei Gao [email protected] Yuzhen Zeng [email protected] Ruolin Mao [email protected] Bo Cui [email protected] Extended author information available on the last page of the article
EPMA Journal
Keywords Induced sputum . Chronic obstructive pulmonary disease (COPD) . Lung function . Metabolomics . Glycerophospholipid metabolism
Data Loading...
