Induction of fungal cellulolytic enzymes using sugarcane bagasse and xylose-rich liquor as substrates
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ORIGINAL PAPER
Induction of fungal cellulolytic enzymes using sugarcane bagasse and xylose‑rich liquor as substrates Sidnei Emilio Bordignon1 · Priscila da Silva Delabona2 · Deise Lima2 · Olavo Perrone1 · Márcia Gleice da Silva Souza3 · Alberdan Silva Santos3 · José Geraldo da Cruz Pradella2 · Maurício Boscolo1 · Eleni Gomes1 · Roberto da Silva1 Received: 28 February 2019 / Revised: 25 November 2019 / Accepted: 8 January 2020 © Associação Brasileira de Engenharia Química 2020
Abstract Liquor from pretreatment of sugarcane bagasse is a potential substrate for multiple purposes due to the high concentration of residual sugars. Nevertheless, several potentially toxic byproducts are also present. However, a few microorganisms are able to overcome this toxicity by growing on these liquors. Twenty-five filamentous fungi were evaluated in submerged cultivation, but none was able to grow using liquor at a concentration of 100% as the liquid medium. However, six fungi were selected for enzyme induction after being grown in diluted liquor at 50% (v/v) using two feed pulses. Induction experiments were performed using 1% untreated and pretreated sugarcane bagasse. FPase and xylanase activities were detected for all six fungi in submerged cultivation, whereas β-glucosidase was observed in four fungi. The highest xylanase activity (28.8 IU mL−1) was at 72 h for T. harzianum P49P11 using pretreated-SCB as an inducer. This work showed a successful alternative for the final destination of liquor residue as substrate for fungi cultivation prior to enzyme production. Keywords Cellulase · Filamentous fungi · Pretreatment · Lignin · Biomass
Introduction An important concept related to efficient processing of renewable feedstock into bio-based products is the “biomass biorefinery”, which aims to convert lignocellulosic biomass into intermediate outputs (cellulose, hemicellulose, lignin) to be processed into a spectrum of products and bioenergy (Cherubini et al. 2009). In this context, a biorefinery must maximize biomass use, generating energy and chemicals of interest with minimum gas emissions and waste (Jong and Jungmeier 2015). For cellulosic ethanol production (2G
* Sidnei Emilio Bordignon [email protected] 1
Laboratory of Biochemistry and Applied Microbiology, São Paulo State University-UNESP, IBILCE, 2265 Cristóvão Colombo, São José Do Rio Preto, São Paulo 15054‑000, Brazil
2
Brazilian Bioethanol Science and Technology Laboratory, CTBE, Polo II de Alta Tecnologia, 10000 Giuseppe Máximo Scolfaro, Campinas, SP 13083‑100, Brazil
3
Laboratory of Systematic Investigation in Biotechnology and Molecular Biodiversity, Federal University of Pará (UFPA), Augusto Correa, Belém, PA 66075‑110, Brazil
ethanol) from sugarcane bagasse, pretreatment is practically compulsory to reduce crystallinity of cellulose and open up the overall structure of biomass by depolymerization and solubilization of hemicelluloses (Alvira et al. 2010). Pretreatment characteristics should include: low cost, possibility to use on an industrial scale,
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