Inhibition Effects of Celery Seed Extract on Human Stomach Cancer Cell Lines Hs746T
The inhibition of tumor cell growth without toxicity in normal cells has attracted attention as an important target in cancer therapy. As a food and drug, Celery Seed Extract (CSE) has been shown its anti-tumour effect by inhibition of tumor promotion and
- PDF / 294,325 Bytes
- 8 Pages / 439.37 x 666.142 pts Page_size
- 9 Downloads / 166 Views
Inhibition Effects of Celery Seed Extract on Human Stomach Cancer Cell Lines Hs746T Lin-Lin Gao, Chang-Xiang Zhou, Xiu-Feng Song, Ke-Wei Fan and Fu-Rong Li
Abstract The inhibition of tumor cell growth without toxicity in normal cells has attracted attention as an important target in cancer therapy. As a food and drug, Celery Seed Extract (CSE) has been shown its anti-tumour effect by inhibition of tumor promotion and inducement of tumor cell apoptosis, but its mechanism and relation with the expression of gap junction protein is still not well known. The present study was designed to evaluate the relationship between inhibition of expression of gap junction protein Connexin 43 (Cx43) and anti-tumor effect of CSE on human stomach cancer cell lines Hs746T. Methods: The human stomach cancer cell line Hs746T was used in the study. The effects of CSE on Connexin 43 were examined by RT-PCR, western blot and immunofluorescence; cell growth and proliferation was examined by the 3-(4,5-dimethylthiazol-2-yl)2,5-diphenyl-tetrazolium bromide (MTT) assay; cell apoptosis was determined by apoptosis related protein bcl-2 and bax. Results: CSE inhibited the growth and proliferation on Hs746T; CSE increased the expression of Cx43 mRNA and protein; CSE decreased Bcl-2 and increased Bax on Hs746T cells. Conclusion: This study firstly shows that CSE can increase Cx43 expression in mRNA and protein level, and exerts its anti-tumour effects on stomach cancer Hs746T cells via inhibiting cell proliferation and inducing cell apoptosis. Keywords Celery seed extract Proliferation
Connexin 43
Apoptosis
Tumour
There is significant evidence that GJIC is important in at least some prodrug/ suicide gene systems by augmenting the bystander effect (BE). GJIC is made up of connexins (Cxs), among which Cx43 is present in most tissues. Stomach cancer is one of the most frequent digestive system cancers and the leading cause of death among malignancies in most of country in the world [1]. Recent evidence suggests L.-L. Gao (&) C.-X. Zhou X.-F. Song K.-W. Fan F.-R. Li Atherosclerosis Institute, Department of Pathology and Pathophysiology, Taishan Medical University, Taian, People’s Republic of China e-mail: [email protected]
S. Li et al. (eds.), Frontier and Future Development of Information Technology in Medicine and Education, Lecture Notes in Electrical Engineering 269, DOI: 10.1007/978-94-007-7618-0_319, Springer Science+Business Media Dordrecht 2014
2553
2554
L.-L. Gao et al.
that apoptosis of cells is closely related to the occurrence, progress and metastasis of tumors [2]. Study of the mechanisms of apoptosis in tumor cells is an important field of tumor therapy and molecular cancer biology [3, 4]. Celery or Apium graveolens L (Fam. Umbelliferae), is widely grown in the temperate zone as a garden crop, and its leaf stalks are used as a popular vegetable in most of Asian countries and extract of celery has been recommended in Chinese herbal medicine as a remedy for hypertension and the ‘‘cleansing’’ of blood [5]. Gap
Data Loading...
